This study uses FRET-based imaging to measure real-time cGMP levels in arterioles of live mice, showing PDEs affect signaling duration but not amplitude, and ACh does not induce endothelial NO release.

bioRxiv · May 30, 2026 · PreprintPhysiology

Pharmacological Activation of NO-Sensitive Guanylyl Cyclase Ameliorates Obesity-Induced Arterial Stiffness

Budbazar Enkhjargal, Aylin Balmes, Danielle Elliott, Lisette Peres Tintin, Timo Kopp, Susanne C. Feil, Robert Feil, Tilman E. Schäffer, Francesca Seta

This study shows that activating NO-sensitive guanylyl cyclase with cinaciguat reduces obesity-induced arterial stiffness by decreasing VSMC cytoskeletal actin polymerization.

Vascular Pharmacology · Feb 23, 2025Peer-reviewedPharmacology & Toxicology

cGMP signaling: probing antagonistic cyclic nucleotide platelet signals by modeling and experiment

Tim Breitenbach, Nils Englert, Özge Osmanoğlu, Natalia Rukoyatkina, Gaby Wangorsch, Andreas Friebe, Elke Butt, Robert Feil, Marcus T. Dittrich, Stepan P. Gambaryan, Thomas Dandekar

The paper develops and validates a model of cGMP signaling and VASP phosphorylation in platelets using data-driven approaches and experimental validation.

bioRxiv · Feb 1, 2021 · PreprintSystems Biology

Dephosphorylation of the NPR2 guanylyl cyclase contributes to inhibition of bone growth by fibroblast growth factor

Leia C. Shuhaibar, Jerid W. Robinson, Giulia Vigone, Ninna Shuhaibar, Jeremy R. Egbert, Valentina Baena, Tracy F. Uliasz, Deborah Kaback, Siu‐Pok Yee, Robert Feil, Melanie C. Fisher, Caroline N. Dealy, Lincoln Ross Potter, Laurinda A. Jaffe

The paper shows that dephosphorylation of NPR2 reduces cyclic GMP, counteracting bone elongation induced by FGF signaling.

eLife · Dec 4, 2017Peer-reviewedDevelopmental Biology