Hanhui Ma

This study improves prime editing efficiency and flexibility by introducing tethered and split pegRNAs, achieving 2-4-fold higher mutation rates in cell lines.

The paper shows that CTCF's DNA binding domain forms dynamic clusters that interact with insulator proteins and avoid transcriptional activators, revealing a mechanism for its insulation function.

This study uses EpiGo-KRAB to introduce H3K9me3 at specific loci and shows it forms heterochromatin-like domains without widespread gene repression.

The paper develops a CRISPR-based system to image epigenetic modification effects on genome organization in living cells and shows it induces genomic relocation and H3K9me3 decoration.

The paper uses CRISPR-Sirius to track chromosomal loci dynamics in living cells and finds cell cycle-dependent changes in DNA contortion and movement.

The paper re-analyzes WGS data from two studies to find that CRISPR off-target mutations include both SNVs and indels, challenging previous conclusions about their rarity.