Miranda E. Pitt

Interrogating the Escherichia coli epitranscriptome via CRISPR interference and Nanopore native RNA sequencing

This study uses CRISPR interference and Nanopore sequencing to investigate RNA modifications in E. coli, revealing their impact on growth and protein pathways.

Viruses, Proviruses and Satellites from Asgard Archaea Enrichments Reveal Complex Microbial Interactions

The paper identifies viruses and proviruses associated with Asgard archaea and reveals virus-mediated interactions with bacteria, expanding understanding of microbial symbiosis in eukaryogenesis.

An Asgard archaeon from a modern analog of ancient microbial mats

The paper describes a novel Asgard archaeon and its syntrophic interaction with a sulfate-reducing bacterium, suggesting insights into early eukaryotic symbiosis.

Profiling of Burkholderia pseudomallei variants derived from Queensland clinical isolates

The paper profiles Burkholderia pseudomallei variants from clinical isolates to understand colony morphotype variation (CMV) using proteomics and genomics, finding that CMV is driven by regulatory mechanisms rather than genomic mutations.

Co-isolation of genetically distinct Burkholderia pseudomallei strains from a single patient in North Queensland

The paper identifies two genetically distinct Burkholderia pseudomallei strains co-isolated from a single patient and presents their multi-omics characterization.

Characterisation of in vitro resistance selection against second-/last-line antibiotics in methicillin-resistant Staphylococcus aureus

This study evaluates in vitro resistance development in MRSA to vancomycin, daptomycin, and linezolid, identifying mutations and mechanisms associated with resistance.

Customizable host and viral transcript enrichment using CRISPR-Cas9 long-read sequencing for isoform discovery and validation

This study uses CRISPR-Cas9 to enrich specific transcripts for long-read sequencing, enabling efficient and accurate isoform discovery and low abundance transcript detection.

MERITS: a web-based integrated MycobacterialPE/PPE protein database

MERITS provides a comprehensive database of PE/PPE proteins from Mycobacterium, offering structural and functional information to aid in vaccine and diagnostic development.

Genomic neighbor typing for bacterial outbreaksurveillance

This study introduces Sketchy, a genomic neighbor typing tool for bacterial outbreak surveillance, showing improved lineage prediction but limitations in clade-specific genotype resolution.

Evaluating the Genome and Resistome of Extensively Drug-Resistant Klebsiella pneumoniae using Native DNA and RNA Nanopore Sequencing

This study compares DNA and RNA nanopore sequencing for detecting drug resistance in XDR K. pneumoniae, finding RNA sequencing identifies more resistance genes and shows strong correlation with qRT-PCR.