Debnath Ghosal

Structural basis for curvature generation and functional specialization in spirochete flagella

The paper determines the near-atomic structures of spirochete flagellar filaments, revealing how sheath proteins generate curvature and enable functional specialization.

Whole cryo-FIB lamella acquisition at high magnification with rectangular beam montage cryo-electron tomography

This paper introduces a method for high-resolution cryo-electron tomography of large biological samples using rectangular beam montage, improving dose efficiency for yeast and human neuron specimens.

The Spatial Architecture of Human Subgingival Biofilms

This study defines the spatial architecture of human subgingival biofilms using fluorescence in situ hybridization and microscopy, revealing structured microbial consortia associated with periodontitis.

Viruses, Proviruses and Satellites from Asgard Archaea Enrichments Reveal Complex Microbial Interactions

The paper identifies viruses and proviruses associated with Asgard archaea and reveals virus-mediated interactions with bacteria, expanding understanding of microbial symbiosis in eukaryogenesis.

Bacterial nanotubes extend from a tubeosome organelle to establish an intercellular bridge

This study visualizes bacterial nanotube formation in Bacillus subtilis, revealing a novel membranous organelle called the tubeosome that facilitates intercellular communication.

A Novel Pilus System in Candidate Phyla Radiation Bacteria

This study identifies two novel pilins, PamA and PamB, from CPR bacteria using cryo-EM, revealing unique structural and assembly features distinct from known pilus systems.

A Tripartite Co-culture System Reveals Defensive Mutualism Between a Nanobdellati Symbiont and Its Host

The paper establishes a tripartite co-culture system to study defensive mutualism between a Nanobdellati symbiont and its host, revealing glycan-mediated protection against viral infection.

An Asgard archaeon from a modern analog of ancient microbial mats

The paper describes a novel Asgard archaeon and its syntrophic interaction with a sulfate-reducing bacterium, suggesting insights into early eukaryotic symbiosis.

A Protein Antibiotic Inhibits the BAM Complex to Kill Without Cell Entry

This study shows that L-type pyocins inhibit the BAM complex at the cell surface to kill Pseudomonas aeruginosa without entering the cell.

Near-atomic structure of the PorKN rings, disulfide bonded to PorG and bound to Attachment Complexes, provide mechanistic insights into the type IX secretion system

The paper presents a cryo-EM structure of the PorKN rings and shows their interaction with PorG and Attachment Complexes, proposing a mechanism for protein secretion in the Type IX Secretion System.

Mutational analysis of the F plasmid partitioning protein ParA reveals novel residues required for oligomerisation and plasmid maintenance

This study identifies mutations in ParA that disrupt polymerization and plasmid maintenance, highlighting the role of ParA oligomerization in plasmid partitioning.

Novel cell-to-cell interactions revealed by cryotomography of a DPANN coculture system

This study uses cryo-ET to reveal intercellular nanotubes between a DPANN archaeon and its host, providing insights into host-DPANN communication and proteomic changes during symbiosis.

A large attachment organelle mediates interaction between a novel Nanobdellota archaeon YN1 and its host

This study isolates and characterizes a novel Nanobdellota archaeon and its host, revealing an attachment organelle that mediates their ectosymbiotic interaction.

Stable sub-complexes observed in situ suggest a modular assembly pathway of the bacterial flagellar motor

The paper observes stable sub-complexes in bacterial flagellar motors using cryo-tomography, suggesting a modular assembly pathway involving independent outer- and inner-membrane sub-complexes.

The structural complexity of the Gammaproteobacteria flagellar motor is related to the type of its torque-generating stators

The paper uses cryo-electron tomography to analyze flagellar motor structures in three Gammaproteobacteria species, finding a correlation between stator type and structural complexity.

Polar targeting and assembly of the Legionella Dot/Icm type IV secretion system (T4SS) by T6SS-related components

This study identifies DotU and IcmF as proteins mediating polar targeting and assembly of the Legionella Dot/Icm T4BSS, revealing an 'outside-inside' biogenesis mechanism.

Molecular architecture of the Legionella Dot/Icm type IV secretion system

The paper describes the molecular architecture of the Legionella Dot/Icm T4BSS using cryo-electron tomography, revealing a central channel and secretion chamber with 13-fold symmetry.