Nelson Castilho Coelho

Environmental stress and phenotypic tradeoff modulate the adaptive potential of novel coding sequences for de novo gene birth

The paper shows that environmental stress influences the phenotypic impact of de novo ORFs, with tradeoffs and stress-dependent growth effects observed in yeast.

Yeast de novo proteins integrate into cellular systems using ancient protein targeting and degradation pathways

This study investigates how de novo proteins in yeast integrate into cellular systems using ancient targeting and degradation pathways, revealing a conserved molecular signature that enables their ER localization and regulation.

A vast evolutionarily transient translatome contributes to phenotype and fitness

The paper identifies a large noncanonical translatome in yeast, showing most translated elements are not conserved genes and contribute to phenotype through fast-evolving mechanisms.

On the illusion of auxotrophy: met15Δ yeast cells can grow on inorganic sulfur thanks to the previously uncharacterized homocysteine synthase Yll058w

The paper shows that yeast cells lacking Met15 can assimilate inorganic sulfur via Yll058w, but require exogenous methionine or hydrogen sulfide chelators to grow, challenging the traditional view of MET15 as essential for sulfur assimilation.

LI Detector: a framework for sensitive colony-based screens regardless of the distribution of fitness effects

The paper introduces LI Detector, a framework for sensitive colony-based fitness screens that does not assume normal distribution of fitness effects, enabling detection of both beneficial and deleterious effects.