Luis Martínez‐Sobrido

Mice immunized with N1 neuraminidase in a single cycle infectious virus or in a recombinant nanoparticle vaccine are protected against lethal challenge with highly pathogenic avian influenza H5N1 influenza

This study shows that mice vaccinated with a single-cycle influenza virus or a neuraminidase-decorated nanoparticle are protected from lethal H5N1 influenza challenge.

A bireporter recombinant SARS-CoV-2 Omicron BA.5 for in vitro and in vivo studies

This paper develops a bireporter recombinant SARS-CoV-2 Omicron BA.5 strain for tracking viral infections and testing antivirals and neutralizing antibodies in vitro and in vivo.

Scarless SARS-CoV-2 Genome Engineering and Variant Analysis

This paper develops a scarless genome engineering system for SARS-CoV-2 to study protease inhibitors and drug resistance mechanisms, showing improved antiviral potency when combined with drug efflux inhibitors.

Genome-Scale Codon Deoptimization Enables Attenuation of Rift Valley Fever Virus

This study uses codon deoptimization to create attenuated Rift Valley Fever Virus (RVFV) vaccine candidates, showing varying degrees of attenuation with the dual-segment deoptimized virus as a promising live-attenuated vaccine.

Mutations in SARS-CoV-2 Nsp1 are critical determinants of viral pathogenicity in mice

This study identifies critical residues in SARS-CoV-2 Nsp1 that determine viral pathogenicity in mice by reducing replication and immune response severity.

Enhanced Pathogenicity and Contact Transmissibility of Human-origin Avian Influenza H5N1 Clade 2.3.4.4b Genotype B3.13 Compared to D1.1 in Ferrets

This study compares the pathogenicity and transmissibility of two H5N1 genotypes in ferrets, finding B3.13 is more pathogenic and transmissible than D1.1, while D1.1 acquires adaptive mutations rapidly.

A One-Shot Multivalent Live-Attenuated Candidate Influenza Vaccine against Divergent Zoonotic H5N1 Clades

This paper develops a multivalent live-attenuated influenza vaccine that provides broad protection against multiple H5N1 clades by incorporating HA and NA from representative clades, showing robust immune responses and protection in mice.

Recovery of proofreading-impaired SARS-CoV-2 reveals a mutator phenotype and an ExoN activity threshold for viability

This paper shows SARS-CoV-2 can tolerate reduced proofreading activity but requires a minimal ExoN activity threshold for viability, leading to a mutator phenotype and reduced replication efficiency.

Development and characterization of mouse-adapted recombinant SARS-CoV-2 expressing reporter genes

This paper develops a recombinant SARS-CoV-2 virus expressing reporter genes to track viral infection in wild-type mice, showing it can be used for vaccine and antiviral research without needing transgenic models.

Substrate and target selectivity of 4'-fluoroadenosine against viral and host polymerases

This study investigates the antiviral activity and mechanism of 4'-fluoroadenosine against viral and host polymerases, showing broad-spectrum inhibition and potential for drug development.

Neuraminidase-Based Cross-Protective Immunity against H5N1 Influenza Viruses in Humans

This study shows that NA-specific antibodies are present in human sera and can be boosted by seasonal vaccination against H5N1 influenza viruses.

Identification and characterization of a SARS-CoV-2 Mpro G23 deletion ensitrelvir-resistant mutant

This study identifies and characterizes a SARS-CoV-2 Mpro G23 deletion mutant resistant to ensitrelvir but not to nirmatrelvir or remdesivir.

Coronavirus protein interaction mapping in bat and human cells identifies molecular and genetic switches for immune evasion and replication

A single PA-X mutation in bovine-origin H5N1 influenza virus reduces pathogenicity in mice

This paper identifies a PA-X mutation in H5N1 influenza virus that reduces pathogenicity in mice by modulating immune responses rather than polymerase activity.

SARS-CoV-2 and MERS-CoV disrupt host protein synthesis via nsp1 with differential effects on the integrated stress response

This study compares how SARS-CoV-2 and MERS-CoV nsp1 proteins affect host protein synthesis and the integrated stress response, revealing differential impacts on eIF2 phosphorylation and stress granule formation.

SARS-CoV-2 Nonstructural Protein 3 Remodels the Phosphorylation of Target Proteins via Protein-Protein Interactions

This paper identifies NSP3 as a viral phosphatase that interacts with N protein and IRF3 to modulate host phosphorylation and suppress antiviral signaling during SARS-CoV-2 infection.

The ORF6 accessory protein contributes to SARS-CoV-2 virulence and pathogenicity in the naturally susceptible feline model of infection

This study shows that deleting ORF6 from SARS-CoV-2 reduces virulence and pathogenicity in cats, with decreased immune suppression and reduced viral replication.

Fluorescent-expressing Modified Vaccinia Ankara Encoding T7 RNA Polymerase

This paper develops fluorescent MVA T7 viruses to monitor helper virus infection and improve reverse genetics for negative-stranded RNA viruses.

Prolyl tRNA Synthetase Is Required for Mammarenavirus Multiplication

This paper shows halofuginone inhibits mammarenavirus replication by targeting prolyl-tRNA synthetase, leading to translation inhibition and reduced viral budding.

A cocktail of SARS-CoV-2 spike stem helix domain and receptor binding domain human monoclonal antibodies prevent the emerge of viral escape mutants

This study shows that a combination of antibodies targeting both the SARS-CoV-2 spike protein's receptor binding domain (RBD) and stem helix (SH) domains prevents the emergence of viral escape mutants.

Bioluminescent reporter influenza A viruses to track viral infections

This paper develops recombinant influenza A viruses expressing nanoluciferase for in vitro and in vivo tracking of viral infections, showing they replicate efficiently and can be imaged using IVIS.

Structure and function of a cross-neutralizing influenza neuraminidase antibody that accommodates recent N2 NA Asn245 glycosylation

This study characterizes a broad-neutralizing influenza antibody, 1122A11, showing it neutralizes multiple NA subtypes and adapts to recent glycosylation changes in H3N2 viruses.

Identification of protective human monoclonal antibodies using a K18 hACE2 transgenic mouse SARS-CoV-2 challenge model

This study identifies protective human monoclonal antibodies against SARS-CoV-2 using a K18 hACE2 transgenic mouse model, showing that in vivo testing improves accuracy over in vitro assays.

Targeted degradation of SARS-CoV-2 via the autophagy-lysosome system using chemical mimetics of the N-degron pathway

This study shows that chemical mimetics of the N-degron pathway, called ATLs, can degrade SARS-CoV-2 via autophagy-lysosome system in cells and animals.

A Human H5N1 Influenza Virus Expressing Bioluminescence for Evaluating Viral Infection and Identifying Therapeutic Interventions

This paper develops a bioluminescent H5N1 influenza virus to track infection and test antiviral drugs in vitro and in vivo.

A luminescent attenuated SARS-CoV-2 for the identification and validation of drug-resistant mutants

This paper develops an attenuated SARS-CoV-2 virus expressing nanoluciferase to identify and validate drug-resistant mutants to nirmatrelvir without using wild-type virus.

Targeting ROCK Signaling Mitigates Influenza Virus-Induced Fibrogenesis in Human Airway Organoids

This study shows that inhibiting the ROCK signaling pathway reduces influenza virus-induced fibrosis in human airway organoids.

Deletion of a KSF Motif Attenuates NSP1 Host Cell Translation Shutoff and Impairs SARS-CoV-2 Virulence

This study shows that a deletion in the NSP1 protein of SARS-CoV-2 variants reduces virulence by impairing host translation shutoff, suggesting NSP1 as a target for antiviral strategies.

Structural Insights into the Assembly and Regulation of 2'-O RNA Methylation by SARS-CoV-2 nsp16/nsp10

This study presents a 2.4 Å crystal structure of the SARS-CoV-2 nsp16/nsp10 complex bound to Cap-1 RNA, revealing structural insights into its assembly and methylation mechanism.

Enhanced RNA replication and pathogenesis in recent SARS-CoV-2 variants harboring the L260F mutation in NSP6

This study identifies the L260F mutation in NSP6 as enhancing RNA replication and pathogenesis in recent SARS-CoV-2 variants, independent of Spike function.