Harald F. Hess

The paper releases a complete larval Drosophila CNS connectome with automated segmentations and highlights the need for improved machine vision for proofreading.

Focal dopaminergic axon swellings in the dorsolateral striatum of Synj1 R259Q mutant mice are clusters of DAT-rich, onion-like plasma membrane invaginations, and the same region shows reduced evoked dopamine release.

This study identifies miR-34/449 miRNAs as regulators of choroid plexus multiciliogenesis, showing their role in controlling cerebrospinal fluid production through modulation of Shh signaling.

The paper introduces image analysis tools for SEM images, available as Fiji plugins and Python scripts, for quality assessment and compatibility with custom FIB-SEM instruments.

The paper introduces an alternating angle milling technique to reduce streaking artifacts in FIB-SEM imaging, improving structural data quality by minimizing non-uniform material removal.

The paper combines brain-wide activity imaging with electron microscopy to identify a distributed neural network for processing threatening somatosensory stimuli in Drosophila larvae.

This study uses cross-species connectomics to show that olfactory circuits have conserved core elements and species-specific rewiring linked to ecological differences.

PATHFINDER improves neuron reconstruction accuracy and efficiency in mouse brain connectome mapping by reducing error rates and manual effort.

The paper presents the first 3D reconstruction of an insect compound eye using vEM, revealing cellular structure and specialized photoreceptors in the parasitoid wasp Megaphragma viggianii.

A FIB-SEM connectome of the male Drosophila right optic lobe sorts ∼53,000 neurons into 727 types, with neurotransmitter predictions and 577 matched split-GAL4 lines.

This study shows that multicilia in the choroid plexus transduce non-canonical Shh signaling to regulate CSF production by repressing Aqp1 and Atp1a2 expression.

This study uses single-particle tracking and 3D FIB-SEM to reveal dynamics and organization of individual vimentin filaments within dense networks, showing bidirectional motion and minimal local co-alignment with microtubules.

The paper identifies periodic ER-PM junctions in dendrites that enable long-range Ca2+ signal integration through local spine activation and ryanodine receptor-dependent Ca2+ release.

The paper describes an automated ultra-microtome for TEM sectioning, achieving high success rates in cutting and picking up sections from various biological samples.

Roughly 23 thousand traced neurons and 10 million TBars of the male Drosophila nerve cord, reconstructed from electron microscopy, typed, and queryable in neuPrint down to motor neurons.

The paper introduces methods to prepare Drosophila brains for high-throughput FIB-SEM connectomics, enabling faster and more accurate imaging of neural connections.

The paper identifies linked chromatin domains (CDs) with nanoscale functional topography in somatic cells, showing cohesin and histone modifications are confined to surfaces and enriched in cores, independent of cohesin.

This paper introduces enhanced FIB-SEM technology that significantly increases imaging volume and resolution, enabling large-scale connectomics and cell biology studies.

The paper introduces GCIB-SEM, a 3D electron microscopy technique that provides <10 nm isotropic resolution for large volumes, improving z-resolution and compatibility with multibeam SEMs.

The paper shows that astrocytes detoxify fatty acids from hyperactive neurons through mitochondrial oxidation, protecting neurons from toxicity.

The paper combines expansion and lattice light sheet microscopy to enable high-speed, nanoscale molecular imaging of neural circuits across large volumes.