Author
Howard Hughes Medical InstituteORCID 0000-0002-3917-6965Unclaimed ORCID profile · follow to get notified of new preprints
8 papers
Recent·Most cited
Christian Arthur, Daniel Edward Milkie, Andrew J. Ulmer, Justin Patrick Ellis, Hark Kyun Kim, Feifei Song, Alejandro Chavez, Wesley R. Legant
This paper introduces a reconfigurable microscopy module that combines HIST and line-scanning confocal techniques for versatile fluorescence imaging.
bioRxiv · Sep 16, 2026 · PreprintBioengineering
Tian-ming Fu B, Gaoxiang Liu, Daniel Edward Milkie, Xiongtao Ruan, Frederik Görlitz, Yu Shi, Valentina Ferro, Nikita S. Divekar, Wei Wang, Harrison M. York, Velat Kilic, Matthew Mueller, Yajie Liang, Timothy A. Daugird, María José Gacha Garay, , Srigokul Upadhyayula
The paper introduces MOSAIC, a multimodal adaptive optical microscope that enables non-invasive imaging across biological scales in live specimens.
Nature Methods · May 22, 2026Peer-reviewedCell Biology
Wei Wang, Xiongtao Ruan, Gaoxiang Liu, Daniel Edward Milkie, Wenping Li, Eric Betzig, Srigokul Upadhyayula, Ruixuan Gao
The paper develops photochemical sectioning to enable nanoscale volumetric imaging of whole-mount specimens, demonstrating its application in analyzing axon degeneration and myelination in mouse olfactory bulbs.
Microscopy and Microanalysis · Oct 16, 2025Peer-reviewedNeuroscience
Xiongtao Ruan, Matthew Mueller, Gaoxiang Liu, Frederik Görlitz, Tian-ming Fu B, Daniel Edward Milkie, Joshua L. Lillvis, Alexander Kühn, Johnny Gan Chong, Jason Li Hong, Chu Yi Aaron Herr, Wilmene Hercule, Marc Nienhaus, Alison N. Killilea, Eric Betzig, Srigokul Upadhyayula
The paper introduces PetaKit5D, a software solution for efficient processing of petabyte-scale light sheet microscopy data, achieving performance improvements over existing methods.
Nature Methods · Oct 17, 2024Peer-reviewedBioinformatics
Ahmed S. Abdelfattah, Jihong Zheng, Amrita Singh, Yi-Chieh Huang, Daniel Reep, Getahun Tsegaye, Arthur Tsang, Benjamin J. Arthur, Monika Řehořová, Carl V.L. Olson, Yichun Shuai, Lixia Zhang, Tian-ming Fu B, Daniel Edward Milkie, Maria V. Moya, , Ilya Kolb
The paper identifies a mutation that enhances the sensitivity of a fluorescent voltage indicator, improving its ability to detect both spiking and subthreshold voltage signals in multiple organisms.
Neuron · Apr 3, 2023Peer-reviewedNeuroscience
Joshua L. Lillvis, Hideo Otsuna, Xiaoyu Ding, Igor Pisarev, Takashi Kawase, Jennifer I. Colonell, Konrad Rokicki, Cristian Goina, Ruixuan Gao, Amy Hu, Kaiyu Wang, John Bogovic, Daniel Edward Milkie, Linus Meienberg, Brett D. Mensh, , Barry J. Dickson
The paper develops a method to rapidly reconstruct neural circuits with single synapse resolution using tissue expansion and lattice light sheet microscopy, validated in Drosophila.
eLife · Oct 26, 2022Peer-reviewedNeuroscience
Ruixuan Gao, Shoh M. Asano, Srigokul Upadhyayula, Igor Pisarev, Daniel Edward Milkie, Tsung‐Li Liu, Ved P. Singh, Austin R. Graves, Grace H. Huynh, Yongxin Zhao, John Bogovic, Jennifer I. Colonell, Carolyn M. Ott, Christopher T Zugates, Susan J. Tappan, , Eric Betzig
The paper combines expansion and lattice light sheet microscopy to enable high-speed, nanoscale molecular imaging of neural circuits across large volumes.
Science · Jan 17, 2019Peer-reviewedNeuroscience
Zhihao Zheng, J. Scott Lauritzen, Eric Perlman, Camenzind G. Robinson, Matthew Nichols, Daniel Edward Milkie, Omar Torrens, John Price, Corey B. Fisher, Nadiya Sharifi, Steven A. Calle-Schuler, Lucia Kmecová, Iqbal J. Ali, Bill Karsh, Eric T. Trautman, , Davi D. Bock
The paper provides a complete electron microscopy volume of the adult Drosophila melanogaster brain, revealing new insights into synaptic connectivity and neuronal circuitry.
Cell · Jul 1, 2018Peer-reviewedNeuroscience