Nature MethodsNeurosciencePeer-reviewed journal article
An optimized acetylcholine sensor for monitoring in vivo cholinergic activity
Miao JingiD, Yuexuan LiiD, Jianzhi ZengiD, Pengcheng HuangiD, Miguel SkirzewskiiD, Ornela KljakiciD, Wanling PengiD, Tongrui QianiD, Ke TaniD, Jing ZouiD, Simon TrinhiD, Runlong WuiD, Shichen ZhangiD, Sunlei Pan, Samuel Andrew HiresiD, Min XuiD, Haohong LiiD, Lisa M. SaksidaiD, Vânia F. PradoiD, Timothy J. BusseyiD, Marco A. M. PradoiD, Liangyi CheniD, Heping ChengiD, Yulong LiiD
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- 408
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- top 1%
Abstract
The ability to directly measure acetylcholine (ACh) release is an essential first step towards understanding its physiological function. Here we optimized the GRABACh (GPCR-Activation-Based-ACh) sensor with significantly improved sensitivity and minimal downstream coupling. Using this sensor, we measured in-vivo cholinergic activity in both Drosophila and mice, revealing compartmental ACh signals in fly olfactory center and single-trial ACh dynamics in multiple regions of the mice brain under a variety of different behaviors
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