Philippe C Després

The paper uses CRISPR screening to identify secretory pathway genes that drive antifungal tolerance through aneuploidy in Candida albicans.

The paper shows that resistance mutations to antifungal drugs are conserved across fungal species but epistasis limits their transferability.

This study uses pooled CRISPRi screening in Candida albicans to identify dosage-sensitive genes across diverse environments and genetic backgrounds, highlighting potential antifungal targets.

The paper curates AMR mutations in fungi to create FungAMR, identifies universal resistance mutations, and provides a tool to screen genomes for AMR mutations.

This paper uses CRISPR-Cas9 to induce targeted loss of heterozygosity in Candida albicans and validates its role in antifungal resistance.

The paper duplicates and evolves an homodimeric enzyme to show that compensatory mutations can lead to functional heteromers through subfunctionalization.

BFG-PCA is a high-throughput method for discovering binary protein interactions using DHFR-PCA, showing high sensitivity and specificity across human and yeast protein pairs.

The paper shows that SH3 domain specificity in PPIs is influenced by host protein identity and domain position, not just intrinsic domain properties.

This study uses base editing to systematically mutate over 17,000 sites across 1,500 genes in yeast, identifying 1,100 sites affecting fitness and providing guidelines for effective gRNA design.

beditor designs guide RNA libraries for CRISPR-mediated base editing across species and editors, using scores to select optimal guides for minimizing off-target effects and maximizing editing efficiency.

This paper develops a double selection strategy to improve CRISPR-Cas9 and base editing efficiency in yeast, enabling high-throughput mutagenesis screens.