Rebecca S. Shapiro

Differential requirement for the Ire1 luminal domain in Candida albicans drug susceptibility and pathogenicity

This study shows that the luminal domain of Ire1 in C. albicans is critical for cell wall integrity, thermal stress, and virulence, while the transmembrane domain is sufficient for azole antifungal tolerance.

CRISPR-activation reveals key resistance genes and vulnerabilities of copy number variants in Candida albicans

This study uses CRISPR-activation to identify genes with fitness effects in Candida albicans isolates, revealing resistance genes and vulnerabilities associated with copy number variants.

Chromosome-scale CRISPR screening reveals secretory pathway genes as drivers of aneuploidy-mediated antifungal tolerance

The paper uses CRISPR screening to identify secretory pathway genes that drive antifungal tolerance through aneuploidy in Candida albicans.

The Unique Efg1 Fungal Virulence Regulon in the Catheterized Bladder Environment

This study identifies the Efg1 regulon active in C. albicans during growth in human urine, validates its clinical relevance in catheter samples, and characterizes key downstream factors involved in fungal CAUTI.

Distinct Ire1-driven transcriptional responses control morphogenesis in Candida albicans

This study identifies Ire1-driven transcriptional responses distinct from the canonical UPR in C. albicans, revealing HAC1-independent pathways and RIDD activity in morphogenesis.

Pooled CRISPRi screening reveals fungal-specific vulnerabilities across environments and genetic backgrounds

This study uses pooled CRISPRi screening in Candida albicans to identify dosage-sensitive genes across diverse environments and genetic backgrounds, highlighting potential antifungal targets.

HyperdCas12a-Based Multiplexed Genetic Regulation in Candida albicans

This paper introduces a hyperdCas12a-based CRISPR system for multiplexed gene regulation in Candida albicans, demonstrating its effectiveness in studying genetic interactions and drug resistance.

FungAMR: A comprehensive portrait of antimicrobial resistance mutations in fungi

The paper curates AMR mutations in fungi to create FungAMR, identifies universal resistance mutations, and provides a tool to screen genomes for AMR mutations.

Targeted loss of heterozygosity in Candida albicans using CRISPR-Cas9

This paper uses CRISPR-Cas9 to induce targeted loss of heterozygosity in Candida albicans and validates its role in antifungal resistance.

A role for the putative error-prone polymerase REV1 in DNA damage and antifungal drug resistance in Candida albicans

This study investigates the role of REV1, an error-prone polymerase, in DNA damage and antifungal drug resistance in Candida albicans, finding that its deletion increases mutation rates and resistance.

Evolutionary diversity of the control of the azole response by Tra1 across yeast species

The paper investigates how Tra1 regulates azole resistance in different yeast species, finding species-specific effects on gene expression and drug tolerance.