Christopher Esk

The paper uses CRISPR-LICHT to screen SOX2 targets in human brain organoids, identifying 28 new genes involved in neural progenitor lineage expansion and survival.

GTestimate improves scRNA-seq normalization by using the Good-Turing estimator to better estimate relative gene expression and cell-cell distances.

The paper evaluates four organoid protocols to recreate human brain cell diversity, identifies factors affecting variability, and introduces a scoring system to assess differentiation propensities.

The paper explains the clonal composition of neural organoids using a neutral competition model that accounts for observed lineage size variability.

The paper uses CRISPR and single-cell transcriptomics to screen ASD risk genes in brain organoids, identifying developmental defects and regulatory modules linked to autism.

This study identifies the HUSH complex as an epigenetic regulator of protocadherin expression in neurons, affecting brain development and neuronal diversity in mice and humans.

The paper identifies a stress signature in brain organoid scRNA-seq data and introduces Gruffi to remove stressed cells, improving developmental accuracy.