Alfredo De Biasio

Structural basis for aphidicolin inhibition of human processive DNA synthesis

This study reveals the structural mechanism of aphidicolin inhibition of human DNA polymerases Pol δ and Pol ε, showing how it blocks DNA synthesis by altering the polymerase conformation.

Structural basis of nick translation in human DNA replication

This study presents cryo-EM structures of human DNA replication complexes to elucidate the structural basis of nick translation during Okazaki fragment maturation.

Structure of the human CTF18-RFC clamp loader bound to PCNA

This study presents a cryo-EM structure of the human CTF18-RFC complex bound to PCNA, revealing structural features important for PCNA loading and Pol ε-mediated leading strand synthesis.

PAF15 stabilizes PCNA on DNA and slows down its sliding dynamics

This study shows PAF15 stabilizes PCNA on DNA, slows its sliding, and regulates its loading/unloading, impacting genome stability.

Structural visualisation of the dynamics of DNA unwinding by a replicative helicase

This study uses cryo-EM to visualize the dynamics of DNA unwinding by the SV40 Large Tumor Antigen helicase, revealing how ATP hydrolysis drives translocation and strand separation.

Cryo-EM structure of Pol κ-DNA-PCNA holoenzyme and implications for polymerase switching in DNA lesion bypass

The paper determines Cryo-EM structures of Pol κ-DNA-PCNA and Pol δ-DNA-PCNA complexes to elucidate the mechanism of polymerase switching during DNA lesion bypass.