Author

Robert A. Nicholas

University of North Carolina at Chapel HillORCID 0000-0003-2507-8974Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Microbiology· 3 Biochemistry· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:human· 3 Assay:whole-genome sequencing· 2 Research Focus:chromatin & epigenetic regulation· 1 Research Focus:drug response / drug discovery· 1 Research Focus:host-pathogen & immune response· 1 Organism:non-human primate· 1 Research Focus:protein folding & dynamics· 1

Frequent Co-Authors

Adriana Le VaniD· 2 shared Ann E. JerseiD· 2 shared Christopher Davies· 2 shared Marissa M. Bivins· 2 shared Sandeepchowdary BalaiD· 2 shared Tatum D. MortimeriD· 2 shared Yonatan H. GradiD· 2 shared Aditi MukherjeeiD· 1 shared

Activity by Year

2025-2026 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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2 shared papersMicrobiology

Adriana Le VanORCID

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4 papers

Recent·Most cited

BiochemistrybioRxivPreprintAug 10, 2026

Energetic coupling of an active site residue in penicillin-binding protein 2 from Neisseria gonorrhoeae with a resistance-associated conformational switch in the β3-β4 loop

Caleb M. Stratton, Sandeepchowdary Bala, Marissa M. Bivins…·Christopher Davies

This study investigates how Tyr422 in PBP2 couples ligand recognition to conformational changes in the β3-β4 loop, revealing energetic coupling mechanisms underlying ceftriaxone resistance in Neisseria gonorrhoeae.

DOI 10.64898/2026.08.07.743578

non-human primateprotein structure

𝕏 PostBluesky

MicrobiologybioRxivPreprintNov 13, 2025

Antibiotic-resistance mutations in penicillin-binding protein 2 from the ceftriaxone-resistant Neisseria gonorrhoeae strain H041 strike a delicate balance between increasing resistance and maintaining transpeptidase activity

Marissa M. Bivins, Joshua Tomberg, Madeleine Bagshaw…·Robert A. Nicholas

This study identifies key mutations in PBP2 from a ceftriaxone-resistant Neisseria gonorrhoeae strain that balance resistance and transpeptidase activity.

DOI 10.1101/2025.11.13.688206

humanproteomics

𝕏 PostBluesky

MicrobiologybioRxivPreprintJun 27, 2025

The role of the L421P mutation in Penicillin-Binding Protein 1 (PBP1) in the evolution of chromosomally mediated penicillin resistance in Neisseria gonorrhoeae

Gabriella L. Gentile, Bryan B. Guzmán, Adriana Le Van…·Robert A. Nicholas

This study shows that the ponAL421P mutation in PBP1 increases penicillin MIC above the clinical breakpoint and persists in N. gonorrhoeae despite penicillin's removal from treatment guidelines.

DOI 10.1101/2025.06.27.662027

humanwhole-genome sequencing

𝕏 PostBluesky

MicrobiologybioRxivPreprintFeb 3, 2025

Quantifying the impact of antibiotic use and genetic determinants of resistance on bacterial lineage dynamics

David Helekal, Tatum D. Mortimer, Aditi Mukherjee…·Yonatan H. Grad

This study quantifies how antibiotic use and genetic resistance determinants affect bacterial lineage dynamics using a Bayesian model and Neisseria gonorrhoeae isolates.

5 citations

humanwhole-genome sequencing

𝕏 PostBluesky