Author

Henry L. Puhl

National Institutes of HealthORCID 0000-0003-3095-7201Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Neuroscience· 2 Bioengineering· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:mouse· 3 Research Focus:cell type annotation· 2 Assay:fluorescence / live-cell microscopy· 1 Assay:imaging· 1 Research Focus:synaptic & neural circuits· 1

Frequent Co-Authors

David M. LovingeriD· 3 shared Ao DongiD· 2 shared Daniel J. LiputiD· 2 shared Kaikai HeiD· 2 shared Yulong LiiD· 2 shared Abigail M HolderiD· 1 shared Aurora Sheridan· 1 shared Barna DudokiD· 1 shared

Activity by Year

2021-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 27 mutual co-author links

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3 shared papersNeuroscience

David M. LovingerORCID

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3 papers

Recent·Most cited

Split intein vectors permit oversize FLIM-FRET biosensor neuronal expression

Jeong Oen Lee, Henry L. Puhl, Abigail M Holder, Aurora Sheridan, Caleb Darden, Muhammad Shah, William Dunne, Tuan Nguyen, Kirk Hines, Shana M. Augustin, Youngchan Kim, Steven S. Vogel, David M. Lovinger

The paper develops a split intein strategy to deliver oversized FLIM-FRET biosensors for neuronal cAMP monitoring in behaving mice.

bioRxiv · Jun 5, 2026 · PreprintBioengineering

2-Arachidonoylglycerol mobilization following brief synaptic stimulation in the dorsal lateral striatum requires glutamatergic and cholinergic neurotransmission

Daniel J. Liput, Henry L. Puhl, Ao Dong, Kaikai He, Yulong Li, David M. Lovinger

This study investigates the mechanisms of 2-AG mobilization in the dorsal lateral striatum following synaptic stimulation, revealing the roles of glutamatergic and cholinergic neurotransmission.

Neuropharmacology · Dec 8, 2021Peer-reviewedNeuroscience

A fluorescent sensor for spatiotemporally resolved endocannabinoid dynamics in vitro and in vivo

Ao Dong, Kaikai He, Barna Dudok, Jordan S. Farrell, Wuqiang Guan, Daniel J. Liput, Henry L. Puhl, Ruyi Cai, Huan Wang, Jiali Duan, Eddy Albarran, Jun Ding, David M. Lovinger, Bo Li, Iván Soltész, Yulong Li

The paper introduces a genetically encoded fluorescent sensor, GRABeCB2.0, to measure endocannabinoid dynamics with high spatiotemporal resolution in vitro and in vivo, revealing new insights into eCB signaling in neurons and brain regions.

Nature Biotechnology · Nov 11, 2021Peer-reviewedNeuroscience