Paolo Annibale

Subcellular pharmacology of a novel cell-permeable fluorescent phosphodiesterase inhibitor using advanced fluorescence microscopy

The paper develops a fluorescent PDE4 inhibitor to visualize drug-target interactions in living cells using advanced fluorescence microscopy techniques.

Effects of altered gravity on adrenergic-mediated cAMP signalling in intact cells

The paper investigates how altered gravity affects adrenergic-mediated cAMP signaling in intact cells, finding shifts in cAMP production EC50 and kinetics under hypergravity and microgravity.

PERK retains a predominantly monomeric state under ER stress conditions

This study uses fluorescence microscopy to show PERK remains mostly monomeric under ER stress, challenging the idea that oligomerization is required for its activation.

MRAP2 modifies signaling and oligomerization state of the melanocortin-4 receptor

This study shows that MRAP2 modifies MC4R signaling and oligomerization, enhancing G protein signaling while reducing beta-arrestin2 recruitment and receptor internalization.

The ratio between experimental and theoretical localization precision of single molecules is sensor-dependent and typically larger than one

The paper examines the discrepancy between theoretical and experimental localization precision in single-molecule imaging, finding that experimental precision is often higher than theoretical estimates.

Shedding Light on the D1-Like Receptors: A Fluorescence-Based Toolbox for Visualization of the D1 and D5 Receptors

The paper introduces a fluorescent ligand for visualizing D1 and D5 dopamine receptors, showing high affinity and selectivity with applications in fluorescence microscopy.

Fluorescent Tools for Imaging and Ligand Screening of Dopamine D2-Like Receptors

This paper describes the synthesis and characterization of fluorescent ligands for imaging and ligand screening of dopamine D2-like receptors, showing high affinity and selectivity.

Fluorescence spectroscopy of low-level endogenous β-Adrenergic Receptor expression at the plasma membrane of differentiating human iPSC-derived cardiomyocytes

The paper uses fluorescence spectroscopy to track β1/2-AR membrane abundance in differentiating hiPSC-derived cardiomyocytes and compares it to mRNA levels in WT and knock-in clones.

Visualization of class A GPCR oligomerization by image-based fluorescence fluctuation spectroscopy

This study uses image-based fluorescence fluctuation spectroscopy to assess oligomerization of Class A GPCRs in intact cells, finding that β2-AR shows slight oligomerization while others are predominantly monomeric.