Allison Grace Rothrock

Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function

This paper develops a genome engineering workflow for primary human NK cells using CRISPR/Cas9 and AAV6 to integrate and disrupt genes, enhancing anti-tumor function through targeted CAR expression.

Spleen Tyrosine Kinase (SYK) negatively regulates ITAM-mediated human NK cell signaling and CD19-CAR NK cell efficacy

This study shows that SYK negatively regulates NK cell signaling and that its deletion enhances CD19-CAR NK cell efficacy in cancer immunotherapy.

Scalable intracellular delivery via microfluidic vortex shedding enhances the function of chimeric antigen receptor T-cells

This study introduces hydroporation as a scalable and efficient method for delivering CAR-T cells, achieving higher yields and viability compared to traditional methods.

Ultra-high efficiency T cell reprogramming at multiple loci with SEED-Selection

This paper develops a method to efficiently reprogram T cells at multiple loci using SEED-Selection, achieving high purity for single and combined gene edits.