Stefan Legewie

The paper shows that intron retention and alternative exon choice are coupled through multistep exon recognition by the spliceosome, which is disrupted in cancer.

This study shows that anaphase synchrony in fission yeast depends on rapid securin degradation and is limited by molecular noise, without requiring positive feedback.

The paper investigates how RNA G-quadruplexes enable cooperative binding of HNRNPH and regulate splicing, showing clinical relevance in breast cancer.

CRISPRgate improves loss-of-function screening by combining CRISPRko and CRISPRi to enhance gene repression and reduce noise in genetic studies.

The paper uses kinetic modeling and statistical learning to quantify how mutations affect splicing outcomes, revealing context-dependent isoform changes and cryptic splice site activation.

The paper links TGFβ signaling, gene expression, and cellular fate decisions in mammary epithelial cells, identifying transcriptional regulators that ensure specific responses at high TGFβ doses.

The paper identifies mutations and RNA-binding proteins affecting CD19 splicing and CART-19 therapy resistance through high-throughput mutagenesis and mathematical modeling.

The paper identifies factors contributing to technical variability between bulk and single-cell RNA-seq experiments and introduces FAVSeq, a machine learning pipeline to detect these factors.

The paper analyzes high-throughput mutagenesis data to show that exon definition is crucial for reliable alternative splicing in the RON proto-oncogene.