Julian König

This study identifies FMR1 as a translational enhancer of oskar in Drosophila oocytes and reveals how its RNA-binding domains regulate opposing translation functions through phase separation.

The paper introduces iCLIP3, a streamlined protocol for mapping RBP-RNA interactions at single-nucleotide resolution, with improvements in visualization, RNA isolation, and multiplexing.

The paper shows that intron retention and alternative exon choice are coupled through multistep exon recognition by the spliceosome, which is disrupted in cancer.

The paper investigates the RNA binding roles of Rrm4's RRM domains in Ustilago maydis, revealing how different RRM interactions influence mRNA transport and stability.

The paper investigates how RNA G-quadruplexes enable cooperative binding of HNRNPH and regulate splicing, showing clinical relevance in breast cancer.

The paper identifies an SLBP-independent mechanism for maintaining maternal histone mRNA stability in C. elegans oogenesis, mediated by the PETISCO complex and TOST-1.

The paper identifies RNA-dependent proteins in cell division using R-DeeP, revealing new interactions and phosphorylation events involving AURKA, KIFC1, and TPX2.

The paper uses kinetic modeling and statistical learning to quantify how mutations affect splicing outcomes, revealing context-dependent isoform changes and cryptic splice site activation.

This study develops a microbial iCLIP2 protocol to identify RNA-protein interactions in fungi, revealing Rrm4 binds mitochondrial mRNA stop codons.

This study uses long-read transcriptome sequencing to analyze SF3B1 mutations in CLL and MDS patients, revealing common splicing alterations and their impact on disease prognosis.

This paper introduces a rapid ribosome profiling protocol for low-input samples, reducing RNA input to 0.1 pmol and enabling high-quality data from small samples.

This study shows that PURA depletion affects posttranscriptional regulation and P-body formation, linking PURA Syndrome to neurodevelopmental disorders.

The paper identifies mutations and RNA-binding proteins affecting CD19 splicing and CART-19 therapy resistance through high-throughput mutagenesis and mathematical modeling.

The paper introduces an improved CLIP protocol (iiCLIP) that produces more specific RNA binding site datasets compared to existing methods.

The paper improves m6A RNA modification detection using miCLIP2 and m6Aboost, identifying thousands of high-confidence sites in human and mouse cells without requiring Mettl3 depletion.

This study shows that the m6A reader Ythdf limits axonal growth in Drosophila by modulating Fmr1 target selection.

The paper analyzes high-throughput mutagenesis data to show that exon definition is crucial for reliable alternative splicing in the RON proto-oncogene.

This study shows that Makorin1 promotes oskar translation by displacing Bruno from its 3' UTR, essential for embryonic patterning and germ cell formation in Drosophila.

This study identifies MKRN1 as a key player in ribosome-associated quality control by promoting ribosome stalling at poly(A) sequences to prevent erroneous protein production.

The paper uses spliceosome iCLIP to map human spliceosome engagement with snRNAs and pre-mRNAs, identifying over 50,000 branchpoints and their associated splicing factor binding patterns.

The paper identifies GTSF-1 as a protein required for the formation of an RNA-dependent RNA Polymerase complex in C. elegans, which is essential for 26G-RNA generation.