Complete protection by a single dose skin patch delivered SARS-CoV-2 spike vaccine
C. L. D. McMillan, J. J. Y. Choo, A. Idris, A. Supramaniam, N. Modhiran, A. A. Amarilla, A. Isaacs, S. T. M. Cheung, B. Liang, H. Bielefeldt-Ohmann, A. Azuar, D. Acharya, G. Kelly, G. J. P. Fernando, M. J. Landsberg, A. A. Khromykh, D. Watterson, P. R. Young, N. A. J. McMillan, D. A. Muller
Abstract
SARS-CoV-2 has infected over 160 million people and resulted in more than 3.3 million deaths, and we still face many challenges in the rollout of vaccines. Here, we use the high-density microarray patch to deliver a SARS-CoV-2 spike subunit vaccine directly to the skin. We show the vaccine, dry-coated on the patch is thermostable, and delivery of spike via HD-MAP induced greater cellular and antibody immune responses, with serum able to potently neutralize clinically relevant isolates including those from the B.1.1.7 and B.1.351 lineages. Finally, a single dose of HD-MAP-delivered spike provided complete protection from a lethal virus challenge, demonstrating that HD-MAP delivery of a SARS-CoV-2 vaccine is superior to traditional needle-and-syringe vaccination and has the potential to greatly impact the ongoing COVID-19 pandemic.
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