The Streptococcus pneumoniae transcriptome in patient cerebrospinal fluid identifies novel virulence factors required for meningitis

Emma CB Wall; José Afonso Guerra‐Assunção; Marie Yang; Rutger Konig; Teerawit Audshasai; Alize M Proust; Rieza Aprianto; Elisa Ramos‐Sevillano; Giuseppe Ercoli; Nicola Bordin; V. S. Agostinho-Terra; Jan‐Willem Veening; David Griffith Lalloo; Brendan William Wren; Robert John Wilkinson; Matthijs C. Brouwer; Diederik van de Beek; Aras Kadioglu; Robert S. Heyderman; Jeremy Brown

doi:10.1101/2024.04.30.591261

MicrobiologybioRxivApr 30, 2024

Preprint

The Streptococcus pneumoniae transcriptome in patient cerebrospinal fluid identifies novel virulence factors required for meningitis

Emma CB Wall iD, José Afonso Guerra‐Assunção iD, Marie Yang iD, Rutger Konig, Teerawit Audshasai iD, Alize M Proust iD, Rieza Aprianto iD, Elisa Ramos‐Sevillano iD, Giuseppe Ercoli iD, Nicola Bordin iD, V. S. Agostinho-Terra iD, Jan‐Willem Veening iD, David Griffith Lalloo iD, Brendan William Wren iD, Robert John Wilkinson iD, Matthijs C. Brouwer iD, Diederik van de Beek iD, Aras Kadioglu iD, Robert S. Heyderman iD, Jeremy Brown iD

Abstract

To better understand Streptococcus pneumoniae pathogenesis we performed RNA sequencing on cerebrospinal fluid (CSF) from meningitis patients to identify bacterial genes expressed during invasion of the central nervous system. Comparison to transcriptome data for serotype 1 S. pneumoniae cultured in ex vivo human CSF defined a subset of 57 genes with high expression during human meningitis. Deletion of two of the most highly expressed genetic loci, bgaA (encodes for a {beta}-galactosidase) or the SP_1801-5 putative stress response operon, resulted in S. pneumoniae strains still able to transmigrate the blood brain barrier but which were more susceptible to complement opsonisation and unable to maintain brain infection in a murine meningitis model. In 1144 meningitis patients, infection with bgaA containing S. pneumoniae strains was associated with a higher mortality (22% versus 14% p=0.02). These data demonstrate that direct bacterial RNAseq from CSF can identify previously undescribed S. pneumoniae virulence factors required for meningitis pathogenesis.

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