Nature CommunicationsSynthetic BiologyPeer-reviewed journal article
Multiplex base editing to convert TAG into TAA codons in the human genome
Yuting CheniD, Eriona HysolliiD, Anlu CheniD, Stephen Casper, Songlei LiuiD, Yi YangiD, Chenli LiuiD, George McDonald ChurchiD
Abstract
Large-scale recoding has been shown to enable novel amino acids, biocontainment and viral resistance in bacteria only so far. Here we extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and examine base-editing genome-wide (observing ~ 40 C-to-T off-target events in essential gene exons). We also introduce GRIT, a computational tool for recoding. This demonstrates the feasibility of recoding, and multiplex editing in mammalian cells.
- Citations
- 24
- FWCI
- 1.55
- Field percentile
- top 17%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Enabling large-scale genome editing by reducing DNA nicking
Cory J. Smith, Oscar Castanon, … George McDonald Church·Nucleic Acids ResearchPeer-reviewed·Apr 1, 2020
- Programmable RNA base editing with a single gRNA-free enzyme
- A single CRISPR base editor to induce simultaneous C-to-T and A-to-G mutations
R. C. Sakata, S. Ishiguro, … N. Yachie·bioRxiv·Aug 8, 2019
- Target-Stabilized Base Editors Enable Robust High-Fidelity RNA Editing
Taian Liu, Yunping Lin, … Zhonghua Lu·Nature CommunicationsPeer-reviewed·Feb 25, 2026
- Preventing escape and malfunction of recoded cells due to tRNA base changes
Anush Chiappino-Pepe, Felix Radford, … George McDonald Church·bioRxiv·Jul 18, 2024
- Development of deaminase-free T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase
Huawei Tong, Haoqiang Wang, … Hui Yang·Nature CommunicationsPeer-reviewed·Jun 8, 2024