Antibiotic Resistance in Bacteria

This paper presents a method to quantify intrabacterial drug accumulation and metabolism in Gram-negative bacteria using liquid chromatography-mass spectrometry, showing correlations with drug efficacy and synergy.

This study profiles antibiotic resistance, virulence genes, and mobile genetic elements in Tibetan antelope gut microbiomes, identifying species-specific resistance and potential human pathogen strains.

The paper evaluates the BD Phoenix CPO Detect assay for detecting OXA-48-producing E. coli without co-producing ESBL/pAmpC, showing high accuracy and rapid results.

This study develops a mechanism-based pharmacokinetic/pharmacodynamic model to evaluate PMB-based combination therapy against CRKP, showing synergistic effects with lower PMB doses.

This study analyzes carbapenem-resistant hypervirulent Klebsiella pneumoniae strains in China, identifying resistance mechanisms and risk factors for ceftazidime/avibactam.

This study shows that the AcrAB-TolC efflux pump has strain-specific roles in drug resistance, biofilm formation, and virulence in Klebsiella pneumoniae.

This study identifies IS1216 as a key driver in the evolution of pRUM-like multidrug resistance plasmids in Enterococcus faecium through comparative genomic analysis of 152 plasmid sequences.

This study identifies an adjuvant, JNAL-016, that inhibits SaErmC-mediated resistance in E. coli, restoring antibiotic efficacy against macrolides and lincosamides.

This study shows that intracellular Acinetobacter baumannii can act as a reservoir for recurrent lung infections by evading immune responses and antibiotics through a 'persist and resist' strategy.

This study investigates whether the rmpADC operon alone can increase virulence in classical Klebsiella pneumoniae strains, finding that HMV is necessary but not sufficient for hypervirulence.

This study presents cryo-EM structures of ArnC, revealing its conformational changes and catalytic mechanism for aminoarabinose glycosylation in polymyxin resistance.

This study investigates collateral sensitivity in bacterial pathogens, finding that resistance mutations can sometimes increase susceptibility to other antibiotics, but these mutations are often costly and not present in clinical isolates.

This study examines allele-specific effects of Rifampin Resistance-Determining Region (RRDR) mutations in RpoB on E. faecium physiology and antibiotic resistance, revealing varied impacts on growth, susceptibility, and hospital survival.

This study identifies evolutionary trade-offs in Acinetobacter baumannii associated with high-level colistin resistance through experimental evolution and genomic analysis.

The paper determines the binding modes of three isoxazolyl penicillins to a class-A beta-lactamase at near physiological temperatures using crystallography.

This study characterizes two multidrug-resistant E. coli strains from bovine mastitis in Bangladesh, revealing their resistome, virulome, and evolutionary relationships.

The paper reports genomic and epidemiological data on a novel Acinetobacter baumannii international clone, IC10, predominantly found in the Middle East.

This study evaluates screening methods to detect ESBL-producing E. coli and K. pneumoniae in healthcare environmental samples, finding that 18-hour pre-enrichment and specific selective agar improve detection.

This study shows that a single conserved residue transfer between RND efflux pump clusters alters drug resistance and conformational dynamics.

amr.watch provides an interactive platform to analyze and visualize AMR trends from global WGS data, enabling ongoing situational awareness for research and policy.

The paper identifies a large number of potential beta-lactamases across diverse bacterial genera and highlights the role of horizontal gene transfer in spreading antibiotic resistance.

The paper identifies a new PAC-2 β-lactamase variant in a K. quasipneumoniae isolate from Colombia conferring resistance to ceftazidime-avibactam.

The paper integrates metagenome-assembled genomes with clinical isolates to identify genomic signatures of K. pneumoniae in carriage and disease, revealing distinct diversity and improved classification using machine learning.

This study investigates how ecological interactions influence the growth of antibiotic-resistant E. coli strains in human gut microbiomes, finding that strain-specific factors and competitive interactions drive their success.

The paper introduces a pipeline using PPINs and machine learning to identify ARGs, showing improved discrimination of ARGs from nonARGs and their association with mobile genetic elements.

This paper shows that disrupting undecaprenyl phosphate recycling via DedA4 deletion reduces AmpC beta-lactamase production and beta-lactam resistance in P. aeruginosa.

This study compares the proteomic responses of imipenem-resistant and wildtype Pseudomonas aeruginosa strains, revealing stable resistance mechanisms in the clinical strain and reactive responses in the control strain.

The paper identifies nitrofurantoin-resistant and pre-resistant E. coli clones in poultry and humans across multiple countries, linking them to historical antibiotic use in poultry farming.

This study investigates the role of MurM and branched cell wall structures in penicillin resistance in Streptococcus pneumoniae, finding no direct correlation between increased branching and resistance.