Transgenic Plants and Applications

Recombinant production of spider silk protein in Physcomitrella photobioreactors

This study successfully produces recombinant spider silk protein containing both N- and C-terminal domains in moss photobioreactors.

Establishing the green algae Chlamydomonas incerta as a platform for recombinant protein production

This paper evaluates Chlamydomonas incerta as a platform for recombinant protein production, showing higher expression levels for mCherry and comparable performance for xylanase and PHL7 compared to C. reinhardtii.

Rhizobium rhizogenes A4-derived strains mediate hyper-efficient transient gene expression in Nicotiana benthamiana and other solanaceous plants

This paper shows that Rhizobium rhizogenes A4-derived strains provide more efficient transient gene expression in Nicotiana benthamiana and other solanaceous plants compared to common agrobacterial strains.

Localising enzymes to biomolecular condensates increases their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana

The paper shows that localizing enzymes to biomolecular condensates in Nicotiana benthamiana increases enzyme stability and improves metabolic pathway yields.

Molecular changes in agroinfiltrated leaves of Nicotiana benthamiana expressing suppressor of silencing P19 and coronavirus-like particles

The paper compares immune responses in Nicotiana benthamiana leaves expressing coronavirus-like particles (CoVLPs) or influenza VLPs, showing overlapping defense responses and demonstrating that Kunitz trypsin inhibitors reduce CoVLP-induced symptoms.

Broad application of plant protein tagging with ALFA tag for nanobody-based imaging and biochemical approaches

The paper evaluates the ALFA tag/ALFA Nanobody technology in plants, demonstrating its versatility for protein detection, interaction studies, purification, and imaging across multiple cellular compartments.

Immunity gene silencing increases transient protein expression in Nicotiana benthamiana

The paper identifies immunity genes that suppress transient protein expression in Nicotiana benthamiana by silencing them and observing increased GFP expression.

Direct Nuclear Delivery of Proteins on Living Plant via Partial Enzymatic Cell Wall Digestion

This study develops a method to deliver proteins into plant nuclei by partially digesting the cell wall, demonstrating efficient GFP delivery in Arabidopsis root cells.

Successful establishment of an exogenous gene expression system in Chlamydomnas chloroplast based on viral replication elements

This study establishes a gene expression system in Chlamydomonas chloroplast using viral replication elements, achieving higher heterologous gene expression than homologous recombination.

CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation.

The paper introduces CuBe, a copper-regulated plant-based expression system for scalable recombinant protein production, demonstrating high eGFP and anti-SARS-CoV-2 antibody expression in Nicotiana benthamiana.

Optimizing Plant Biofactories: Enhancing Recombinant Protein Production in Nicotiana benthamiana through Phytoplasma Effectors

This study enhances recombinant protein production in Nicotiana benthamiana by expressing TENGU without signal peptide, resulting in increased biomass and soluble protein content.

Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant-Specific Insert Domain in Protein Function and Subcellular Localization

This study produces cyprosin B in Nicotiana benthamiana and shows that removing the PSI domain increases enzymatic activity and alters subcellular localization.

Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform

The paper develops a chloroplast-based cell-free gene expression platform across multiple plant species to accelerate synthetic biology research and gene expression studies.

Mobius Assembly for Plant Systems highlights promoter-coding sequence terminator interaction in gene regulation

The paper introduces a new toolkit for plant synthetic biology, MAPS, which reveals promoter-coding sequence terminator interactions affecting gene regulation.

The application of Nicotiana benthamiana as a Transient Expression Host to Clone the Coding Sequences of Plant Genes

This study shows that Nicotiana benthamiana can effectively clone plant gene coding sequences by transcribing and splicing gDNA from certain species.

Glyco-engineered pentameric SARS-CoV-2 IgMs show superior activities compared to IgG1 orthologues

This study expresses glycoengineered pentameric SARS-CoV-2 IgMs in plants, showing 390-fold increased neutralization potency compared to IgG1 orthologues.

Stable expression of Helicobacter pylori cagA oncoprotein in brinjal

The paper expresses the H. pylori cagA oncoprotein in transgenic brinjal plants and confirms its stable expression through molecular and immunological assays.

Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana

This study demonstrates the use of synthetic genetic elements to control and optimize the production of Lepidopteran sex pheromones in Nicotiana benthamiana.

Rational domestication of a plant-based recombinant expression system expands its biosynthetic range.

This study demonstrates the rational domestication of Nicotiana benthamiana to expand its ability to produce stable therapeutic peptides.

Plant-based Production and Characterization of a Promising Fc-fusion Protein against Bone Mass Density Loss

This study produces and characterizes a plant-based PTH-Fc fusion protein that shows similar potency to PTH (1-34) in binding and activating PTH1R, making it suitable for space medicine applications.

Production of recombinant human acid α-glucosidase with mannosidic N-glycans in α-mannosidase I mutant rice cell suspension culture

Production and secretion of functional full-length SARS-CoV-2 spike protein in Chlamydomonas reinhardtii

This study produces functional, full-length SARS-CoV-2 spike protein in Chlamydomonas reinhardtii, a cost-effective alga, and demonstrates its interaction with ACE2.

Mobius Assembly for Plant Systems highlights promoter-terminator interaction in gene regulation

The paper introduces MAPS, a Golden Gate Assembly system for plant synthetic biology, and shows that promoter-terminator interactions significantly affect gene expression.

Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii

This paper reports the production of SARS-CoV-2 spike RBD in algae, showing it can bind ACE2 and may serve as a low-cost antigen source.

Expression and Manufacturing of Protein Therapeutics in Spirulina

This paper develops a genetic engineering platform in spirulina for producing bioactive protein therapeutics at scale, demonstrating its potential for low-cost biopharmaceutical manufacturing.

Pilot production of SARS-CoV-2 related proteins in plants: a proof of concept for rapid repurposing of indoors farms into biomanufacturing facilities

The paper demonstrates the rapid production of SARS-CoV-2 related proteins in plants using Nicotiana benthamiana for potential biomanufacturing applications.

Introduction of a leaky stop codon as molecular tool in Chlamydomonas reinhardtii

The paper introduces a leaky stop codon as a tool to enable simultaneous expression of functional and fluorescently tagged proteins in Chlamydomonas reinhardtii.

Calreticulin co-expression supports high level production of a recombinant SARS-CoV-2 spike mimetic in Nicotiana benthamiana

This study shows that co-expressing human calreticulin in plants enhances the production of a SARS-CoV-2 spike mimetic, supporting plant-based vaccine development.