Transgenic Plants and Applications

Bacteriophage P22 virus-like particles as nanoscale protein scaffolds for plant synthetic biology

This paper demonstrates the use of bacteriophage P22 virus-like particles as nanoscale protein scaffolds for plant synthetic biology, showing their ability to assemble, encapsulate, and display proteins in Nicotiana benthamiana.

Eliminating polyphenol oxidase from Nicotiana benthamiana improves recombinant protein yield and purity and facilitates native-state protein studies

This study shows that eliminating polyphenol oxidase in Nicotiana benthamiana improves recombinant protein yield, purity, and native-state integrity during extraction.

The Arabidopsis CYSTM α 5'UTR increases protein production from transgenes in plants and bacteria.

This study identifies a 79-nucleotide sequence from the Arabidopsis CYSTM1 5'UTR that significantly enhances transgene expression in plants and bacteria.

Causes and consequences of experimental variation in Nicotiana benthamiana transient expression

The paper models variability in Nicotiana benthamiana transient expression and provides guidelines for minimizing it through experimental design and power analysis.

Knocking out two polyphenol oxidase genes significantly improves recombinant protein purification in Nicotiana benthamiana

This study shows that knocking out two polyphenol oxidase genes in Nicotiana benthamiana improves recombinant protein purification by reducing aggregation and increasing yields.

Precision Glycoform Engineering: Combining plant and in vitro systems for tailored biopharmaceutical production

This study combines plant and in vitro glycoengineering to produce homogeneous glycoforms of pharmaceutical proteins efficiently.

Secretion-based production of prolyl-hydroxylated human type III collagen in scalable Physcomitrella photobioreactors

This paper produces prolyl-hydroxylated human type III collagen in moss bioreactors, achieving 1 mg/L in 11 days with no need for heterologous P4H expression.

Clinical-grade Plant-made nanomaterials: from Process Design to the construction of a Manufacturing facility.

This study develops a GMP-compliant manufacturing process for TBSV-based nanoparticles designed to induce immune tolerance in autoimmune diseases and evaluates its scalability and environmental impact.

A high-throughput heterologous expression platform for plant synthetic biology based on Arabidopsis suspension cells

This paper develops a high-throughput platform for transient and stable multigene expression in Arabidopsis suspension cells, enabling efficient protein production for synthetic biology applications.

Monitoring the stability of transgene expression in lettuce using the RUBY reporter.

This study evaluates three transgene expression constructs in lettuce using the RUBY reporter, finding that the LsUBI promoter/terminator provides stable, uniform expression while the AtUBI promoter/tRBCS offers an intermediate option.

Catch & Release - rapid cost-effective protein purification from plants using a DIY GFP-Trap-protease approach

The paper introduces a cost-effective, one-step protein purification method for plants using a DIY GFP-Trap-protease system, enabling rapid isolation of soluble and membrane-bound proteins.

Development of modular geminivirus-based vectors for high cargo expression and gene targeting in plants

The paper develops a modular geminivirus-based vector system for improved protein expression and gene targeting in plants, showing that native promoters and removal of virion-sense genes enhance reporter expression without affecting replicon accumulation.

Production of human papillomavirus type 16 virus-like particles in Physcomitrella photobioreactors

This paper produces and purifies human papillomavirus type 16 virus-like particles in Physcomitrella photobioreactors, achieving high yields and successful assembly.

A versatile protocol for purifying recombinant proteins from Nicotiana benthamiana for structural studies

This paper presents a protocol for purifying recombinant proteins from Nicotiana benthamiana for structural studies, demonstrating its effectiveness for complex purification and cryo-EM analysis.

Expression of a constitutively active nitrate reductase increases SARS-CoV-2 Spike protein production in Nicotiana benthamiana leaves that otherwise show traits of senescence

This study shows that expressing a constitutively active nitrate reductase in Nicotiana benthamiana increases SARS-CoV-2 Spike protein production by reducing leaf senescence and improving nitrogen metabolism.

Recombinant production of spider silk protein in Physcomitrella photobioreactors

This study successfully produces recombinant spider silk protein containing both N- and C-terminal domains in moss photobioreactors.

Establishing the green algae Chlamydomonas incerta as a platform for recombinant protein production

This paper evaluates Chlamydomonas incerta as a platform for recombinant protein production, showing higher expression levels for mCherry and comparable performance for xylanase and PHL7 compared to C. reinhardtii.

Rhizobium rhizogenes A4-derived strains mediate hyper-efficient transient gene expression in Nicotiana benthamiana and other solanaceous plants

This paper shows that Rhizobium rhizogenes A4-derived strains provide more efficient transient gene expression in Nicotiana benthamiana and other solanaceous plants compared to common agrobacterial strains.

Localising enzymes to biomolecular condensates increases their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana

The paper shows that localizing enzymes to biomolecular condensates in Nicotiana benthamiana increases enzyme stability and improves metabolic pathway yields.

Molecular changes in agroinfiltrated leaves of Nicotiana benthamiana expressing suppressor of silencing P19 and coronavirus-like particles

The paper compares immune responses in Nicotiana benthamiana leaves expressing coronavirus-like particles (CoVLPs) or influenza VLPs, showing overlapping defense responses and demonstrating that Kunitz trypsin inhibitors reduce CoVLP-induced symptoms.

Immunity gene silencing increases transient protein expression in Nicotiana benthamiana

The paper identifies immunity genes that suppress transient protein expression in Nicotiana benthamiana by silencing them and observing increased GFP expression.

Direct Nuclear Delivery of Proteins on Living Plant via Partial Enzymatic Cell Wall Digestion

This study develops a method to deliver proteins into plant nuclei by partially digesting the cell wall, demonstrating efficient GFP delivery in Arabidopsis root cells.

CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation.

The paper introduces CuBe, a copper-regulated plant-based expression system for scalable recombinant protein production, demonstrating high eGFP and anti-SARS-CoV-2 antibody expression in Nicotiana benthamiana.

Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform

The paper develops a chloroplast-based cell-free gene expression platform across multiple plant species to accelerate synthetic biology research and gene expression studies.

The application of Nicotiana benthamiana as a Transient Expression Host to Clone the Coding Sequences of Plant Genes

This study shows that Nicotiana benthamiana can effectively clone plant gene coding sequences by transcribing and splicing gDNA from certain species.

Glyco-engineered pentameric SARS-CoV-2 IgMs show superior activities compared to IgG1 orthologues

This study expresses glycoengineered pentameric SARS-CoV-2 IgMs in plants, showing 390-fold increased neutralization potency compared to IgG1 orthologues.

Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana

This study demonstrates the use of synthetic genetic elements to control and optimize the production of Lepidopteran sex pheromones in Nicotiana benthamiana.

Rational domestication of a plant-based recombinant expression system expands its biosynthetic range.

This study demonstrates the rational domestication of Nicotiana benthamiana to expand its ability to produce stable therapeutic peptides.

Production of recombinant human acid α-glucosidase with mannosidic N-glycans in α-mannosidase I mutant rice cell suspension culture

Pilot production of SARS-CoV-2 related proteins in plants: a proof of concept for rapid repurposing of indoors farms into biomanufacturing facilities

The paper demonstrates the rapid production of SARS-CoV-2 related proteins in plants using Nicotiana benthamiana for potential biomanufacturing applications.