Discover, rate, and discuss biology preprints.

This study designs de novo proteins to inhibit E. coli's heme extraction from hemoglobin, demonstrating their effectiveness at low nanomolar concentrations.

This study identifies the biosynthetic pathway of reserpine in Rauvolfia verticillata, revealing key enzymes and mechanisms for its complex structure formation.

This study uses a live-cell NanoBRET assay to analyze how AKT inhibitors bind to different AKT isoforms and how phosphorylation affects inhibitor binding and cell viability.

The paper develops a mass spectrometry pipeline to quantify tubulin polyglutamylation in Trypanosoma brucei flagella, identifying novel glutamylation sites and crosstalk with detyrosination.

This study shows PHRF1's PHD finger binds histone H3 N-terminal region, regulates transcription and RNA splicing, and is essential for DNA damage response.

The paper presents the first crystal structure of a cis-COM domain in NRPSs and shows its role in scaffolding domain architecture for efficient peptide synthesis.

This study shows human pol δ holoenzymes can bypass various lesions with insertion and extension efficiencies ranging from 0 to 100%.

This study identifies Plasmodium aspartyl-tRNA synthetase as an antimalarial target by showing how natural products inhibit protein translation through reaction hijacking.

The paper uses FRET and MD simulations to determine the conformation of Cav1 intramembrane helices in bicelles, supporting a hairpin structure.

This paper identifies p43 as a lipid-binding protein secreted by Trichuris whipworms that modulates host immune responses by interacting with signaling lipids.

This study uses solid-state NMR to reveal five structural forms of α-glucans in Cryptococcus cell walls, showing their role in scaffolding and interacting with chitin, capsule, and melanin.

This paper develops a synthetic method to create sulfoglucuronyl glycosphingolipids with HNK-1 epitopes, demonstrating their role in complement-mediated erythrocyte lysis by anti-HNK-1 antibodies.

The paper reports the crystal structure of a GABARAP~ATG3 conjugate, revealing a non-covalent interaction between GABARAP and the backside of ATG3's E2 catalytic center, which is important for PE conjugation.

This study identifies a ubiquitin-binding domain in SPRTN that enhances its proteolytic activity towards polyubiquitinated DNA-protein crosslinks.

This paper identifies TrmTS as a tRNA methyltransferase that catalyzes 2'-O-methylation at position 6 in tRNA, extending knowledge of tRNA methyltransferase architecture.

This paper presents a high-throughput screening platform combining TRIC and TR-FRET to identify SLIT2 binders, finding bexarotene as a potent inhibitor with a KD of 2.62 μM.

This study uses stable isotope analysis of dentine to reconstruct the dietary changes and life history of an 18th-century priest from Croatia, linking them to seminary admission timing.

This study characterizes the CDNF-BiP interaction structurally and functionally, revealing CDNF's role as an antagonist of BiP nucleotide exchange and its neuroprotective effects.

This paper characterizes the biochemical properties of glycerol kinase from Haloferax volcanii, highlighting its robustness under extreme conditions and unique kinetic behavior.

This study traces the evolutionary origins of mammalian PRPS genes and shows how their duplication and specialization enable functional multimeric complexes with regulatory features.