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The paper identifies CRISPR-Cas12a variants with expanded PAM recognition, enabling targeting of ~25% of the human genome.

This study identifies 57 uranyl-binding proteins in Arabidopsis thaliana cells, with GRP7 showing specific U(VI) binding and interference with RNA binding, suggesting a role in uranium toxicity.

The paper identifies new enzymatic activities of GroEL/Hsp60 proteins, including thioesterase and esterase functions, and links these to palmitoylation and antibiotic resistance in M. tuberculosis.

This paper introduces an antibody-based system for post-translational modification of endogenous proteins in complex environments, demonstrating its use on green fluorescent protein and beta-2 microglobulin.

This paper introduces FilmCPI, a sequence-based model that leverages data imbalance to improve compound-protein interaction prediction, showing better performance and generalization across datasets.

This study shows that TDP-43 and Tau directly interact, promoting co-condensation but reducing Tau fibril formation and seeding.

The paper identifies a mutation in GGPPS that disrupts inter-dimer interactions, leading to increased substrate affinity and product release, revealing product inhibition as a regulatory mechanism in GGPPS.

The paper determines ambient temperature structures of the bifunctional Nmar_1308 enzyme from Nitrosopumilus maritimus, revealing new conformational dynamics relevant to synthetic biology.

This study identifies sCMF231, a small molecule that activates YAP by inhibiting FUBI-mediated fubylation of Annexin A2, thereby enhancing YAP transcriptional activity.

This study uses cryo-EM to reveal how microtubule association induces an Mg-free apo-like ADP pre-release conformation in kinesin-1, independent of tail interactions.

This study analyzes acetylation across 48 organisms, identifying conserved acetylation sites and their roles in metabolic processes and stress response.

This paper describes the discovery of S-892216, a potent SARS-CoV-2 3CLpro inhibitor with antiviral activity against multiple variants and low cross-resistance to existing drugs.

The paper reports the structure of the PSI-SOD supercomplex from Chromera velia, showing how energy conversion and oxidative stress protection are directly coupled.

The paper introduces and analyzes Q-scores to assess the reliability of atomic coordinate models fitted to 3DEM maps, showing their correlation with map resolution and model quality.

This study functionally characterizes a tetra-modular LPMO from Enterobacter cloacae, revealing its chitin-cleaving activity and structural features involving copper coordination.

The paper develops a kinetic model of the coronavirus main protease incorporating dimerization and ligand binding, showing how inhibitors and substrates affect enzyme activity differently.

This study identifies a metabolic adaptation in osimertinib-resistant NSCLC cells involving acetate production via a PAA shunt, which supports redox balance and bioenergetics.

This study compares FCoV RNA levels in FIP cats and their housemates across blood, conjunctiva, and feces, finding no difference between groups but higher levels in feces overall.

The paper develops Thin-diaPASEF, an optimized diaPASEF method for single-shot proteomics, achieving high protein identification and coverage in HEK cells and multi-sample analyses.

The paper identifies protein ligands for monoacylglycerol lipids using chemical proteomics in mouse brain and cell lysates, revealing Hippocalcin as a novel ligand involved in calcium sensing.