Takehiko Tosha

Use of photocaged molecular oxygen for time-resolved serial X-ray crystallography

This paper uses photocaged molecular oxygen to trigger oxygen binding in myoglobin crystals and captures structural changes using time-resolved serial X-ray crystallography.

Serial femtosecond crystallography reveals the pH-driven allosteric mechanism of hexamer glargine

This study reveals the pH-driven allosteric mechanism of hexamer glargine using serial femtosecond crystallography and multiscale analyses, showing a structural transition linked to delayed release.

Reciprocal-space mapping of diffuse scattering by serial femtosecond crystallography reveals analog-specific disorder in insulin analogs

This study compares structural heterogeneity of insulin analogs using crystallographic analysis across different temperatures, revealing analog-specific disorder patterns.

Monomer-dimer structural comparison in quinol-dependent nitric oxide reductase reveals a functional basis for superior enzymatic activity in the dimer

The paper compares monomer and dimer structures of NmqNOR to show how dimerization enhances enzymatic activity by stabilizing the active site conformation.

Integrated structural dynamics uncover new modes of B12 photoreceptor activation

The paper describes the photoactivation mechanism of the B12 photoreceptor CarH using structural and spectroscopic methods, revealing unique intermediates that drive tetramer dissociation.

Solubility, Hydration, and Sulphate Coordination in Cubic Insulin Crystals from Esrapid™ Monomers Stabilized in Divalent Anionic Form of Citric Acid

This study investigates how water and anion coordination affect insulin's structure and stability across a pH range, revealing pH-dependent electrostatic effects on protein dynamics and stabilization.

Deciphering the altered conformational states of bifunctional thaumarchaeal crotonyl-CoA hydratase and 3-hydroxypropionyl-CoA dehydratase from Nitrosopumilus maritimus

The paper determines ambient temperature structures of the bifunctional Nmar_1308 enzyme from Nitrosopumilus maritimus, revealing new conformational dynamics relevant to synthetic biology.

Experimental and Computational Insights into the Structural Dynamics of the Fc Fragment of IgG1 Subtype from Biosimilar VEGF-Trap

This study presents near-physiological structures of the Fc fragment from a biosimilar VEGF-Trap and shows structural plasticity through molecular dynamics simulations.

Double crossed? Structural and computational studies of an unusual crosslinked heme in Methylococcus capsulatus cytochrome P460

This study identifies a double crosslink between lysine and heme in Methylococcus capsulatus cytochrome P460 using structural biology and QM/MM simulations.