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363,000+ bioRxiv and medRxiv preprints, including those later published in peer-reviewed journals, across 26 biological disciplines.
Recent preprints
- BioengineeringAdvanced Materials TechnologiesPeer-reviewed
Enhanced Piezoelectric Performance of PVDF-TrFE Nanofibers through Annealing for Tissue Engineering Applications
Maksym Krutko, Holly M. Poling, Maulee Sheth…·Leyla Esfandiari
This study enhances the piezoelectric properties of PVDF-TrFE nanofibers through annealing, showing improved mechanical and biological responses for tissue engineering.
17 citations - BioengineeringScientific ReportsPeer-reviewed
Efficient secretion of a plastic degrading enzyme from the green algae Chlamydomonas reinhardtii
João Vitor Dutra Molino, Barbara Saucedo, Kalisa Kang…·Stephen Patrick Mayfield
This paper demonstrates the efficient secretion of a plastic-degrading enzyme, PHL7, from the green algae Chlamydomonas reinhardtii, showing its ability to degrade PET and bio-based polyurethanes.
21 citationstop 3% - BioengineeringAnalytical ChemistryPeer-reviewed
Paired Analyses of Nuclear Protein Targets and Genomic DNA by Single-Cell Western Blot and Single-Cell PCR
Ana E. Gomez Martinez, Trinh Lam, Amy E. Herr
The paper introduces SplitBlot, a microfluidic single-cell assay that pairs genomic DNA analysis with nucleo-cytoplasmic protein measurements from the same cell.
- BioengineeringbioRxivPreprint
Matrix compliance regulates cellular and nuclear confinement of fibroblasts in tunable protein-based hydrogel microenvironments
Indira Priyadarshani Patra, Saujanya Sabarinath, Shiuly Sarkar…·Shantanu Pradhan
This study investigates how tunable hydrogel properties affect fibroblast morphology and nuclear confinement in 3D microenvironments.
- BioengineeringeLifePeer-reviewed
CRISPR-Edited DPSCs, Constitutively Expressing BDNF Enhance Dentin Regeneration in Injured Teeth
Ji Hyun Kim, Muhammad Imran Irfan, Sreelekshmi Sreekumar…·Seung‐Hyuk Chung
This study shows CRISPR-edited dental pulp stem cells overexpressing BDNF enhance dentin regeneration in injured teeth through inflammation-driven mechanisms.
- BioengineeringAdvanced MaterialsPeer-reviewed
Granular hydrogels as brittle yield stress fluids
Gunnar B. Thompson, Jiye Lee, Krutarth M. Kamani…·Brendan A.C. Harley
This paper quantifies the rheological behavior of granular hydrogels using the Kamani-Donley-Rogers model with Brittility, showing they behave as brittle yield stress fluids with complex transient properties.
13 citations - BioengineeringAdvanced SciencePeer-reviewed
Genetic Manipulation of Mammalian Cells in Microphysiological Hydrogels
Anna C. Jäkel, Dong‐Jiunn Jeffery Truong, Friedrich Christian Simmel
This study demonstrates the use of GelMA hydrogels for 3D cell culture with in situ genetic manipulation and spatially controlled gene expression.
1 citation - BioengineeringApplied and Environmental MicrobiologyPeer-reviewed
High Enzyme Promiscuity in Lignin Degradation Mechanisms in Rhodopseudomonas palustris CGA009
Mark Kathol, Niaz Bahar Chowdhury, Cheryl M. Immethun…·Rajib Saha
This study investigates lignin degradation mechanisms in Rhodopseudomonas palustris using multi-omics and CRISPRi to identify essential pathways and improve sustainable valorization of lignin.
7 citations - BioengineeringACS Applied Materials & InterfacesPeer-reviewed
Controlled release of microorganisms from engineered living materials
Manivannan Sivaperuman Kalairaj, Iris George, Sasha M. George…·Taylor H. Ware
This paper demonstrates a method to release probiotics from hydrogels using mechanical fracture, enabling sustained and controllable release of metabolically active microbes.
19 citations - BioengineeringScientific ReportsPeer-reviewed
Deep-Tissue Two-Photon Brain Imaging Enabled by a Tunable Fiber-Optic Dispersive Wave Generator
Marvin Edelmann, Andreu Matamoros‐Angles, Mohsin Shafiq…·Franz X. Kärtner
This paper introduces a tunable fiber-optic dispersive wave generator for deep-tissue two-photon imaging in mouse brains, achieving high-resolution imaging at depths over 450 micrometers.
- BioengineeringProceedings of the ... American Control Conference/Proceedings of the American Control ConferencePeer-reviewed
Body Fluid Estimation during Standard Ultrafiltration in Chronic Kidney Disease
Rammah M. Abohtyra, Omar Ali Beg
This paper evaluates a method to estimate body fluid and blood volumes during hemodialysis using modeling and prediction algorithms without relying on specific sensors or protocols.
DOI 10.23919/acc63710.2025.11108075 - BioengineeringbioRxivPreprint
Osteoblast-derived Nerve Growth Factor is Required for Skeletal Adaptation to Mechanical Load and the Osteoanabolic Effect of Gambogic Amide in Mice
Ibtesam Rajpar, Eric McLaughlin, Gabriella Fioravanti…·Ryan E. Tomlinson
This study shows that osteoblast-derived NGF is essential for bone adaptation to mechanical load and that gambogic amide requires endogenous NGF for its osteoanabolic effects in mice.
2 citations - BioengineeringbioRxivPreprint
Multicellular model of temozolomide resistance in glioblastoma reveals phenotypic shifts in drug response and migratory potential
Victoria A. Kriuchkovskaia, Ela K. Eames, Sydney A. McKee…·Brendan A.C. Harley
This study uses a 3D hydrogel model to investigate TMZ resistance in GBM by examining drug response and migration in heterogeneous cell populations.
3 citations - BioengineeringBioresource Technology ReportsPeer-reviewed
Impact of the rotational speed and counter electrode configuration on the performance of a rotating disc bioelectrochemical reactor (RDBER) operated as microbial electrolysis cell
Zhizhao Xiao, Max Rümenapf, Max Hackbarth…·Johannes Eberhard Reiner
The paper evaluates how rotational speed and counter electrode placement affect a microbial electrolysis cell's performance, finding increased current density and hydrogen production with moderate speeds and reduced hydrogen shuttling with upper cathode placement.
2 citations - BioengineeringAnalytica Chimica ActaPeer-reviewed
A smartphone-based genetically recombinant whole-cell biosensor for highly sensitive monitoring of polychlorinated biphenyls (PCBs)
Qiangqiang Luo, Faying Zhang, Mengjie Zhang…·Guimin Zhang
This paper develops a genetically engineered whole-cell biosensor using E. coli to detect multiple PCB congeners with high sensitivity, and integrates it with a smartphone for real-time monitoring.
6 citations - BioengineeringPlant Biotechnology JournalPeer-reviewed
Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.)
Nathaniel Butler, Aidan T. Carlson, Colby G. Starker, Daniel F. Voytas
This study demonstrates that viral vectors can enhance leaf transformation in Sorghum bicolor by delivering morphogenic regulators, achieving higher embryonic calli formation rates than conventional vectors.
9 citations - BioengineeringbioRxivPreprint
Innate immunocompetent iNSpheroids: A hiPSC-derived 3D model to study the central nervous system captures an early CNS response to rAAV
Catarina Monteiro Gomes, Gabriela Priscila de Lima Silva, Mafalda M Aleixo…·Catarina Brito
The paper develops a 3D human CNS model from hiPSCs to study immune responses to rAAV gene therapy, finding that iMGL-iNSpheroids show mild, transient inflammation to rAAV9.
DOI 10.1101/2025.07.07.662807 - BioengineeringACS Synthetic BiologyPeer-reviewed
A cyanobacterial screening platform for Rubisco mutant variants
Ute A. Hoffmann, Anna Zoey Schuppe, Axel Knave…·Elton Paul Hudson
The paper develops a cyanobacterial platform to screen Rubisco mutants, identifying a variant with improved thermostability and resilience to detrimental exchanges.
10 citations - BioengineeringJournal of Controlled ReleasePeer-reviewed
Low-frequency ultrasound-mediated blood-brain barrier opening enables non-invasive lipid nanoparticle RNA delivery to glioblastoma
Maya Shumer-Elbaz, Nitay Ad‐El, Yulia Chulanova…·Tali Ilovitsh
This paper demonstrates that low-frequency ultrasound can safely open the blood-brain barrier to deliver lipid nanoparticles (LNPs) containing RNA to glioblastoma tumors in mice.
30 citationstop 2%