Author

D. N. Bolam

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3 Microbiology· 3

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Organism:human· 5 Research Focus:cell type annotation· 4 Assay:proteomics· 3 Research Focus:host-pathogen & immune response· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:chromatin & epigenetic regulation· 1 Assay:cryo-EM· 1

Frequent Co-Authors

A. Basle· 3 shared L. I. Crouch· 3 shared D. I. Spencer· 2 shared P. A. Urbanowicz· 2 shared A. Ali· 1 shared A. Bell· 1 shared A. Campbell· 1 shared A. Gajbhiye· 1 shared

Activity by Year

2019-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 11 mutual co-author links

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3 shared papersBiochemistry

A. Basle

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Carbohydrate-active enzymes from Akkermansia muciniphila breakdown mucin O-glycans to completion

L. I. Crouch, C. R. Bakshani, T. O. Ojuri, B. Pilgaard, J. Holck, R. McInnes, M. Zakhour, M. Kerouedan, E. Newton, D. N. Bolam, R. P. Kozak, S. Cakaj

This study characterizes carbohydrate-active enzymes from Akkermansia muciniphila to show they synergistically degrade mucin O-glycans to the core GalNAc.

bioRxiv · Mar 27, 2024 · PreprintBiochemistry

Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria

Y. Wu, A. Bell, G. H. Thomas, D. N. Bolam, F. Sargent, N. Juge, T. Palmer, E. SEVERI

The paper identifies and characterizes anhydro-sialic acid transporters in bacteria, showing their specific or promiscuous substrate preferences.

bioRxiv · Jan 17, 2024Microbiology

Outer membrane utilisomes mediate oligosaccharide uptake in gut Bacteroidetes

J. B. White, A. Silale, M. C. Feasey, T. Heunis, Y. Zhu, H. Zheng, A. Gajbhiye, S. Firbank, A. Basle, M. Trost, D. N. Bolam, N. A. Ranson, B. van den Berg

The paper identifies utilisomes as stable glycan-utilising complexes in Bacteroides thetaiotaomicron, revealing their structure and mechanism of substrate capture via cryo-EM.

bioRxiv · Aug 15, 2022Microbiology

Plant N-glycan breakdown by human gut Bacteroides

L. I. Crouch, P. A. Urbanowicz, A. Basle, Z.-P. Cai, L. Liu, J. Voglmeir, J. M. Melo Diaz, S. T. Benedict, D. I. Spencer, D. N. Bolam

The paper identifies a pathway for breaking down plant N-glycans in gut Bacteroides and characterizes enzymes involved, with potential applications in allergen modification and therapeutic protein stability.

bioRxiv · Apr 7, 2022Biochemistry

Extensive transfer of genes for edible seaweed digestion from marine to human gut bacteria

N. A. Pudlo, G. Vasconcelos Pereira, J. Parnami, M. Cid, S. Markert, J. P. Tingley, F. Unfried, A. Ali, A. Campbell, K. Urs, Y. Xiao, R. Adams, D. Martin, D. N. Bolam, D. Becher, , E. C. Martens

The paper shows gut bacteria have extensively adapted to digest seaweed polysaccharides through gene transfer from marine bacteria, revealing widespread metabolic plasticity in the human gut microbiome.

bioRxiv · Jun 9, 2020 · PreprintMicrobiology

Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

L. I. Crouch, D. N. Bolam, A. Basle, C. J. Stewart, M. Wuhrer, R. J. Linhardt, M. V. Liberato, C. A. Lamb, F. Zhang, K. Cooke, M. Doona, S. Needham, R. R. Brady, J. E. Berrington, K. Madunic, , R. Glowacki

The paper identifies endo-acting O-glycanases in gut bacteria that initiate mucin breakdown, challenging the current model of exo-acting glycosidases.

bioRxiv · Nov 9, 2019Biochemistry