M. Trost

Mass spectrometry-based proteomic exploration of diverse murine macrophage cellular models

The paper compares proteomic profiles of murine macrophage cell lines to primary and immortalised bone marrow-derived macrophages, identifying J774A.1 as the most similar to primary cells.

Proteomic Analysis Reveals Trilaciclib-Induced Senescence

This paper uses proteomics to show trilaciclib induces senescence in CML and NSCLC cells by stabilizing CDK4/6 and activating autophagy.

Proteomic mapping of macrophages in response to the clearance of apoptotic cells reveals a unique reprogramming profile.

This study uses proteomic analysis to reveal reprogramming in macrophages during efferocytosis, showing an alternatively activated phenotype and secretome-driven reprogramming of naive macrophages.

Bi-functional particles for real-time acidification and proteolysis multiplex assay in macrophages

The paper develops a multiplexed assay to simultaneously monitor phagosome acidification and proteolysis in macrophages using silica beads conjugated to pHrodo and DQ BSA.

A Comparison of Quantitative Mass Spectrometric Methods for Drug Target Identification by Thermal Proteome Profiling

This study compares DIA-based label-free quantification methods with TMT-DDA for thermal proteome profiling, finding DIA approaches are comparable or better for detecting drug target engagement.

BtuB TonB-dependent transporters and BtuG surface lipoproteins form stable complexes for vitamin B12 uptake in gut Bacteroides.

This study identifies stable complexes between BtuB transporters and BtuG lipoproteins in Bacteroides thetaiotaomicron for vitamin B12 uptake via cryoEM, X-ray crystallography, and MD simulations.

Outer membrane utilisomes mediate oligosaccharide uptake in gut Bacteroidetes

The paper identifies utilisomes as stable glycan-utilising complexes in Bacteroides thetaiotaomicron, revealing their structure and mechanism of substrate capture via cryo-EM.

Lysosomal damage drives mitochondrial proteome remodelling and reprograms macrophage immunometabolism

This study shows lysosomal damage leads to mitochondrial proteome changes and metabolic reprogramming in macrophages, affecting immune responses to infections.

Mining the human gut microbiome identifies mycobacterial D-arabinan degrading enzymes

The paper identifies D-arabinan degrading enzymes in the human gut microbiome, including new families of glycoside hydrolases and mycobacterial enzymes involved in cell wall degradation.

The Type 9 Secretion System enables sharing of fungal mannan by human gut Bacteroides

The paper shows that Bacteroides salyersiae uses the Type 9 Secretion System to release manno-oligosaccharides from mannan, which can be used by other Bacteroides species.

The E3 ligase RNF115 regulates phagosome maturation and host response to bacterial infection

This study identifies RNF115 as an E3 ligase that regulates phagosomal maturation and host response to bacterial infection through ubiquitylation.

A MALDI-TOF assay identifies nilotinib as an inhibitor of inflammation in acute myeloid leukaemia

This study identifies nilotinib as an anti-inflammatory drug using a MALDI-TOF assay in AML-derived monocytes, showing it inhibits the p38-MK2/3 signaling axis.

The last battle of Anne of Brittany: solving mass grave through an interdisciplinary approach (paleopathology, anthropobiology, history, multiple isotopes and radiocarbon dating)

This study identifies individuals in two Breton mass graves using interdisciplinary methods, revealing they were soldiers from opposing armies during the 1491 siege of Rennes.

Delayed induction of type I and III interferons mediates nasal epithelial cell permissiveness to SARS-CoV-2

This study shows that delayed type I and III interferon responses in nasal epithelial cells allow SARS-CoV-2 replication, but pre-infection IFN treatment restricts virus and preserves barrier integrity.

Proteomics characterisation of the L929 cell supernatant and its role in BMDM differentiation

The paper characterizes L929 cell supernatant proteome and shows it induces a stronger anti-inflammatory M1 phenotype in BMDMs compared to M-CSF alone.

A novel USP30 inhibitor recapitulates genetic loss of USP30 and sets the trigger for PINK1-PARKIN amplification of mitochondrial ubiquitylation.

The paper identifies a USP30 inhibitor that mimics genetic loss of USP30, revealing its role in regulating mitochondrial ubiquitylation and mitophagy through TOM complex components.

Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation

This study compares macrophage responses to human- and animal-adapted tuberculosis pathogens, identifying factors that drive multinucleated cell formation in bovine macrophages infected with M. bovis.

A membrane-depolarising toxin substrate of the Staphylococcus aureus Type VII secretion system mediates intra-species competition

This paper identifies TspA as a new T7SS substrate in S. aureus, showing it mediates intra-species competition by membrane depolarization and growth inhibition in vivo.

Targeted Proteomic Analysis Reveals Enrichment of Atypical Ubiquitin Chains in Murine Contractile Tissues

The paper quantifies ubiquitin chain types in murine tissues using optimized Ub-AQUA-PRM, revealing tissue-specific differences and enrichment of atypical K33 chains in contractile tissues.

Macrophage Scavenger Receptor 1 mediates lipid-induced inflammation in non-alcoholic fatty liver disease

This study identifies MSR1 as a key mediator of lipid-induced inflammation in NAFLD, showing its role in macrophage activation and potential as a therapeutic target.

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44

This study identifies novel SGK3 substrates, including endosomal proteins, through phosphoproteomic screens and shows SGK3 specifically phosphorylates these proteins at sites distinct from Akt.

Profiling embryonic stem cell differentiation by MALDI TOF mass spectrometry: development of a reproducible and robust sample preparation workflow

The paper develops a reproducible MALDI-TOF MS workflow to profile mouse embryonic stem cell differentiation and identifies unique biomarkers.

Triggering MSR1 promotes JNK-mediated inflammation in IL-4 activated macrophages

The paper identifies that MSR1 signaling via K63-polyubiquitylation activates JNK, promoting a pro-inflammatory switch in IL-4 activated macrophages.

The MALDI TOF E2/E3 ligase assay as an universal tool for drug discovery in the ubiquitin pathway

The paper introduces a MALDI TOF-based assay for high-throughput screening of E2/E3 ligase activity in the ubiquitin pathway, demonstrating its utility for drug discovery.

Thermal proteome profiling of breast cancer cells reveals proteasomal activation by CDK4/6 inhibitor palbociclib

This study uses thermal proteome profiling to show palbociclib induces proteasome stabilization and senescence in breast cancer cells via ECM29 loss, with ECM29 mRNA levels linked to relapse-free survival.