Donald G. Moerman

Identification of essential genes in Caenorhabditis elegans through whole genome sequencing of legacy mutant collections

This study identifies 58 essential genes in C. elegans using whole genome sequencing of maternal-effect lethal and sterile mutants, revealing novel alleles and functional roles in embryonic development and fertilization.

Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans.

The paper adapts peel-1 negative selection to CRISPR-Cas9 genome editing in C. elegans, enabling automated screening-free generation and phenotyping of genome-edited animals.

PhenoMIP: High Throughput Phenotyping of Diverse C. elegans Populations via Molecular Inversion Probes

The paper introduces PhenoMIP, a method using molecular inversion probes to phenotype C. elegans mutant strains, finding that ~80% show decreased fitness compared to N2.

The conserved phosphatase GSP-2/PP1 promotes germline immortality via small RNA-mediated genome silencing during meiosis

This paper identifies a meiosis-specific function of the phosphatase GSP-2 in promoting germ cell immortality through small RNA-mediated genome silencing in C. elegans.

Optimizing guide RNA selection and CRISPR/Cas9 methodology for efficient generation of deletions in C. elegans.

The paper develops an optimized CRISPR/Cas9 protocol for generating deletions in C. elegans, demonstrating high efficiency and minimal off-target effects.

CRISPR-Cas9 human gene replacement and phenomic characterization in Caenorhabditis elegans to understand the functional conservation of human genes and decipher variants of uncertain significance

This paper uses CRISPR-Cas9 in C. elegans to replace human genes and assess their function, showing it can classify disease variants accurately.

MIP-MAP: High-Throughput Mapping of Caenorhabditis elegans Temperature Sensitive Mutants via Molecular Inversion Probes

The paper introduces MIP-MAP, a method to map temperature-sensitive mutations in C. elegans using molecular inversion probes, achieving high-resolution genetic mapping with high-throughput sequencing.