Author

F. L. Nobrega

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 7 Biochemistry· 1 Biophysics· 1

Signature Assays, Organisms & Methods

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Organism:bacteria (other)· 6 Approach:experimental assay· 6 Research Focus:host-pathogen & immune response· 6 Approach:statistical model· 4 Assay:DNA methylation· 2 Assay:whole-genome sequencing· 2 Research Focus:chromatin & epigenetic regulation· 1 Assay:CRISPR screen· 1

Frequent Co-Authors

S. J. Brouns· 4 shared Y. Wu· 3 shared A. C. Haagsma· 2 shared A. R. Costa· 2 shared B. Estrada Bonilla· 2 shared C. Aparicio-Maldonado· 2 shared D. W. Taylor· 2 shared E. V. Koonin· 2 shared

Activity by Year

2019-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 21 mutual co-author links

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4 shared papersMicrobiology

S. J. Brouns

Mutual co-authors in cluster

Author profile →

9 papers

Recent·Most cited

Genomic plasticity is a blueprint of diversity in Salmonella lineages

S. Krishnakant Kushwaha, A. Anand, Y. Wu, H. Leonardo Avila, T. Sicheritz-Ponten, A. Millard, S. Amol Marathe, F. L. Nobrega

This study examines genomic plasticity in Salmonella lineages, revealing non-random integration of pathogenicity genes into regions of genomic plasticity and their regulatory relationships.

bioRxiv · Dec 2, 2023 · PreprintMicrobiology

Kiwa is a bacterial membrane-embedded defence supercomplex activated by phage-induced membrane changes

Z. Zhang, T. C. Todeschini, Y. Wu, R. Kogay, A. Naji, J. Cardenas Rodriguez, R. M. Anandhakrishna, D. Kaganovich, D. W. Taylor, J. P. K. Bravo, M. Teplova, T. Amen, E. V. Koonin, D. J. Patel, F. L. Nobrega

The paper characterizes the Kiwa defence system as a bacterial membrane supercomplex that detects phage-induced membrane changes and inhibits phage replication.

bioRxiv · Feb 26, 2023 · PreprintMicrobiology

Synergistic anti-phage activity of bacterial defence systems

Y. Wu, S. K. Garushyants, A. van den Hurk, C. Aparicio-Maldonado, S. K. Kushwaha, C. M. King, Y. Ou, T. C. Todeschini, M. R. J. Clokie, A. M. Millard, Y. E. Gencay, E. V. Koonin, F. L. Nobrega

The paper investigates bacterial defence systems against phage predation, finding synergistic anti-phage activity and compatibility between systems in different bacterial taxa.

bioRxiv · Aug 21, 2022 · PreprintMicrobiology

Accumulation of defense systems in phage resistant strains of Pseudomonas aeruginosa

A. R. Costa, D. F. van den Berg, J. Q. Esser, A. Muralidharan, H. van den Bossche, B. Estrada Bonilla, B. A. van der Steen, A. C. Haagsma, A. C. Fluit, F. L. Nobrega, P.-J. Haas, S. J. Brouns

The paper shows that Pseudomonas aeruginosa strains with more phage defense systems are more resistant to phages, and these systems often target different phage genera.

bioRxiv · Aug 12, 2022 · PreprintMicrobiology

Class I DISARM provides anti-phage and anti-conjugation activity by unmethylated DNA recognition

C. Aparicio-Maldonado, G. Ofir, A. Salini, R. Sorek, F. L. Nobrega, S. J. Brouns

This study identifies Class 1 DISARM as a system providing anti-phage and anti-conjugation activity through recognition of unmethylated DNA sites.

bioRxiv · Dec 28, 2021 · PreprintMicrobiology

Structural basis for broad anti-phage immunity by DISARM

J. P. Bravo, C. A. Maldonado, F. L. Nobrega, S. J. Brouns, D. W. Taylor

This study determines the cryo-EM structures of the DISARM complex DrmAB and elucidates its mechanism of phage DNA recognition and activation.

bioRxiv · Dec 26, 2021Biochemistry

Members of a highly widespread bacteriophage family are hallmarks of metabolic syndrome gut microbiomes

P. A. de Jonge, K. Wortelboer, T. P. M. Scheithauer, B.-J. H. van den Born, A. H. Zwinderman, F. L. Nobrega, B. E. Dutilh, M. Nieuwdorp, H. Herrema

The paper identifies phage families associated with metabolic syndrome by analyzing gut phageome changes and their relationship to bacterial communities.

bioRxiv · Mar 30, 2021Microbiology

Genomic characterization of four novel bacteriophages infecting the clinical pathogen Klebsiella pneumoniae

B. Estrada Bonilla, A. R. Costa, T. van Rossum, S. Hagedoorn, H. Walinga, M. Xiao, W. Song, P.-J. Haas, F. L. Nobrega, S. J. Brouns

The paper isolates and sequences four novel bacteriophages infecting Klebsiella pneumoniae, revealing low sequence identity to known phages and potential new DNA packaging mechanisms and protein clades.

bioRxiv · Mar 2, 2021Microbiology

CRISPR-based DNA and RNA detection with liquid-liquid phase separation

W. K. Spoelstra, J. M. Jacques, F. L. Nobrega, A. C. Haagsma, M. Dogterom, T. Idema, S. J. J. Brouns, L. Reese

This paper introduces a label-free CRISPR-based method for nucleic acid detection using liquid-liquid phase separation, showing that target sequence cleavage reduces turbidity.

bioRxiv · May 15, 2019Biophysics