F. Sun

SARS-CoV-2 Quasispecies provides insight into its genetic dynamics during infection

The paper analyzes SARS-CoV-2 quasispecies structure using SMRT sequencing, revealing a One-King model with dominant and minor haplotypes and their evolutionary dynamics.

Cystic fibrosis transmembrane conductance regulator (CFTR) inhibition results in mucus accumulation in human airway epithelia Calu-3 cells: Experimental and Machine Learning Studies

This study uses electron microscopy and machine learning to show that CFTR inhibition reduces secretory vesicle swelling and mucin release in Calu-3 cells, leading to mucus accumulation.

Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2

This paper develops a rapid, portable RT-LAMP assay for SARS-CoV-2 detection with performance comparable to RT-PCR, using a smartphone-based reader for point-of-care testing.

Inhibition of SARS-CoV-2 infection (previously 2019-nCoV) by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion

This paper identifies EK1C4, a potent fusion inhibitor against SARS-CoV-2 and other coronaviruses, showing high efficacy in vitro and in vivo.

Amorphous nickel titanium alloy film: a new choice for cryo electron microscopy sample preparation

This paper introduces an amorphous nickel-titanium alloy film as a new supporting substrate for cryoEM, showing improved electron conductivity and reduced beam-induced motion, leading to better particle distribution and resolution.

VHUT-cryo-FIB, a method to fabricate frozen-hydrated lamella of tissue specimen for in situ cryo-electron tomography

The paper introduces VHUT-cryo-FIB, a workflow for preparing frozen-hydrated tissue lamella for cryo-ET, and demonstrates its effectiveness using rat and spinach samples.

Molecular mechanism of mitochondrial phosphatidate transfer by Ups1/Mdm35

This study investigates the molecular mechanism of PA transfer by Ups1/Mdm35 using crystal structures, molecular dynamics simulations, and biophysical assays, revealing conformational changes and regulatory factors.

Cryo-EM structures reveal interactions of S-OPA1 with membrane and changes upon nucleotide binding

The paper uses cryo-EM to study SOPA1's structure and conformational changes upon GTP binding, revealing its unique mechanism in mitochondrial membrane fusion.

New interfaces on MiD51 for Drp1 recruitment and regulation

This study identifies two regions on MiD51 that bind Drp1 and shows that MiD51 dimerization via a disulfide bond is required for Drp1 recruitment, revealing a multi-faceted regulatory mechanism for mitochondrial fission.

In situ protein micro-crystal fabrication by cryo-FIB for electron diffraction

The paper develops a workflow for preparing micro/nano-crystals for MicroED, enabling the study of small macromolecular crystals previously unsuitable for this technique.