G. L. Hughes

Establishment and comparative genomics of a high-quality collection of mosquito-associated bacterial isolates -- MosAIC (Mosquito-Associated Isolate Collection)

The paper establishes a high-quality bacterial isolate collection (MosAIC) from mosquitoes, providing genomic data and phylogenomic analysis to study mosquito-associated bacteria.

Wolbachia-induced inhibition of O'nyong nyong virus in Anopheles mosquitoes is mediated by Toll signaling and modulated by cholesterol

This study shows Wolbachia inhibits Onyong nyong virus in Anopheles mosquitoes via Toll signaling, but cholesterol suppresses this effect by modulating Toll signaling.

Identification and characterization of two CRISPR-Cas systems associated with the mosquito microbiome

The paper identifies and characterizes two CRISPR-Cas systems in a mosquito-associated bacterium, revealing differences in their target sequences and potential roles in defense against plasmids and phages.

Persistence of SARS-CoV-2 and its surrogate, bacteriophage Phi6, on surfaces and in water

This study compares the persistence of SARS-CoV-2 and its surrogate Phi6 on surfaces and in water, finding Phi6 overestimates infectiousness and deposition solutions significantly affect viral persistence.

Cryopreservation of mosquito microbiota for use in microcosm experiments

This study develops and validates a method to cryopreserve mosquito microbiota for use in standardized microcosm experiments, showing improved reproducibility and minimal impact of cryopreservation on bacterial recovery.

Vertical and horizontal transmission of cell fusing agent virus in Aedes aegypti

This study investigates the transmission routes of CFAV in Aedes aegypti, finding vertical and paternal transmission rates and suggesting strategies for arbovirus control.

Lack of robust evidence for a Wolbachia infection in Anopheles gambiae from Burkina Faso

This study re-evaluates Wolbachia infection in Anopheles gambiae from Burkina Faso and finds low prevalence, contradicting earlier reports.

Wolbachia endosymbionts in two Anopheles species indicates independent acquisitions and lack of prophage elements

This study reports two high-density Wolbachia strains in Anopheles species, showing independent acquisitions and limited prophage elements, with genomic analysis revealing metabolic completeness and cytoplasmic incompatibility factors.

Inter-species microbiota transplantation recapitulates microbial acquisition and persistence in mosquitoes

This study successfully transfers microbial communities between mosquito species and within species, demonstrating microbiome transplantation in mosquitoes and its impact on microbiota dynamics.

Protein N-glycosylation is essential for SARS-CoV-2 infection

This study shows that host and viral N-glycosylation are essential for SARS-CoV-2 infection, with inhibition reducing infectivity and virion production.

Inactivation of SARS-CoV-2 on surfaces and in solution with Virusend (TX-10), a novel disinfectant

This study demonstrates that Virusend (TX-10) rapidly inactivates SARS-CoV-2 on surfaces and in solution, reducing viral titre by 4log10 PFU/mL within 1 minute.

Negeviruses reduce replication of alphaviruses during co-infection

This study shows negeviruses reduce replication of alphaviruses during co-infection and demonstrate a method to deliver anti-viral antibody fragments using paratransgenic ISVs.

Evidence of exposure to SARS-CoV-2 in cats and dogs from households in Italy

The paper investigates SARS-CoV-2 exposure in companion animals in Italy, finding that 3.4% of dogs and 3.9% of cats had neutralizing antibodies, with higher prevalence in pets from households with COVID-19 cases.

Methods of inactivation of SARS-CoV-2 for downstream biological assays

This study tests methods to inactivate SARS-CoV-2 for use in lower containment laboratories, finding that heat, detergents, and UV light effectively inactivate the virus.

Microbial interactions in the mosquito gut determine Serratia colonization and blood feeding propensity.

This study investigates how microbial interactions in the mosquito gut affect Serratia colonization and blood feeding behavior in Ae. aegypti.

Gut-associated bacteria invade the midgut epithelium of Aedes aegypti and stimulate innate immunity and suppress Zika virus infection in cells.

This study shows gut bacteria invade mosquito cells, activate immune pathways, and reduce Zika virus infection.

CRISPR/Cas9-mediated gene deletion of the ompA gene in an Enterobacter gut symbiont impairs biofilm formation and reduces gut colonization of Aedes aegypti mosquitoes

This study uses CRISPR/Cas9 to delete the ompA gene in an Enterobacter gut symbiont of Aedes aegypti, showing impaired biofilm formation and reduced gut colonization in adults.

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

The paper identifies novel Wolbachia strains in multiple Anopheles species from Sub-Saharan Africa and assesses their association with Plasmodium infection.

Microbiome interaction networks and community structure from lab-reared and field-collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquito vectors.

The paper analyzes microbiome interactions in mosquitoes to identify co-occurrence and co-exclusion patterns across species and environments.

Global transcriptome analysis of Aedes aegypti mosquitoes in response to Zika virus infection

This study analyzes the transcriptome of Aedes aegypti mosquitoes infected with Zika virus at 2, 7, and 14 days post-infection, identifying changes in transcripts and long intergenic non-coding RNAs.