J. Chamot-Rooke

A multi-parameter optimization in middle-down analysis of monoclonal antibodies by LC-MS/MS

This paper optimizes parameters for middle-down analysis of monoclonal antibodies using LC-MS/MS to improve sequence coverage.

Shigella ipaA mediates actin bundling through diffusible vinculin oligomers with activation imprint

This study shows that Shigella IpaA induces vinculin homo-oligomerization, leading to actin bundling and stable cell adhesion independent of force transduction.

TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics

TDFragMapper evaluates experimental parameters in top-down proteomics by visualizing fragment ions and sequence coverage.

Universal amyloidogenicity of patient-derivedimmunoglobulin light chains

This study shows that all tested patient-derived immunoglobulin light chains have comparable amyloidogenic potential, with proteolytic cleavage being a key determinant of in vivo aggregation.

Structural and molecular determinants for the interaction of ExbB from Serratia marcescens and HasB, a TonB paralog

The paper determines the cryo-EM structures of ExbBSm and ExbB-ExbDSm complex, identifies key residues for function and interaction with TonB/HasB, and reveals the role of ExbBSm extension in heme acquisition.

ipaA triggers vinculin oligomerization to strengthen cell adhesion during Shigella invasion

This paper shows that Shigella's IpaA protein causes vinculin to oligomerize, strengthening cell adhesion during bacterial invasion.

The Flemmingsome reveals an ESCRT-to-membrane coupling required for completion of cytokinesis

The paper identifies a plasma membrane-to-ESCRT module essential for abscission, showing its role in cytokinesis, exosome formation, and HIV budding.

Crippling the bacterial cell wall molecular machinery

This study identifies the role of lytic transglycosylase LtgA in bacterial cell wall integrity and shows that targeting it can impair Neisseria meningitidis fitness during infection.