Author

J. D. Puglisi

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 5 Biochemistry· 3

Signature Assays, Organisms & Methods

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Research Focus:cell signaling & trafficking· 7 Approach:experimental assay· 5 Organism:human· 4 Organism:yeast· 4 Assay:fluorescence / live-cell microscopy· 3 Approach:biophysical modeling· 2 Approach:microscopy & imaging analysis· 2 Assay:proteomics· 2

Frequent Co-Authors

J. Wang· 7 shared C. P. Lapointe· 5 shared A. G. Johnson· 3 shared J. Choi· 3 shared A. Prabhakar· 2 shared B.-S. Shin· 2 shared C. Alvarado· 2 shared I. S. Fernandez· 2 shared

Activity by Year

2018-2021 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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7 shared papersBiophysics

J. Wang

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Author profile →

8 papers

Recent·Most cited

eIF5B and eIF1A remodel human translation initiation complexes to mediate ribosomal subunit joining

C. P. Lapointe, R. Grosely, M. Sokabe, C. Alvarado, J. Wang, E. Montabana, N. Villa, B.-S. Shin, T. E. Dever, C. S. Fraser, I. S. Fernandez, J. D. Puglisi

This study uses single-molecule techniques and cryo-EM to show how eIF1A and eIF5B remodel initiation complexes to facilitate ribosomal subunit joining during translation initiation.

bioRxiv · Dec 9, 2021Biophysics

Rapid 40S scanning and its regulation by mRNA structure during eukaryotic translation initiation

J. Wang, C. Alvarado, B.-S. Shin, J. Bohlen, T. E. Dever, J. D. Puglisi

The paper directly observes yeast 43S ribosomal scanning along mRNA 5'UTRs, revealing its speed and structural regulation mechanisms.

bioRxiv · Dec 8, 2021Biophysics

Multiplexed single-molecule analysis of human telomerase synthesizing DNA

J. Hentschel, J. Choi, C. R. Bagshaw, C. P. Lapointe, J. Wang, L. I. Jansson, M. Badstubner, J. D. Puglisi, M. D. Stone

This paper develops a real-time single-molecule RNA sequencing method to visualize telomerase catalysis and structural dynamics at single-nucleotide resolution.

bioRxiv · Jun 25, 2021 · PreprintBiophysics

Mechanisms that ensure speed and fidelity in eukaryotic translation termination

M. R. Lawson, L. N. Lessen, J. Wang, A. Prabhakar, N. C. Corsepius, R. Green, J. D. Puglisi

The paper uses single-molecule fluorescence assays to study eukaryotic translation termination dynamics in yeast, revealing a conserved multi-step process involving eRF1 and eRF3.

bioRxiv · Apr 1, 2021Biochemistry

Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation

C. P. Lapointe, R. Grosely, A. G. Johnson, J. Wang, I. S. Fernandez, J. D. Puglisi

This paper investigates how SARS-CoV-2 NSP1 inhibits translation initiation by competing with mRNA for binding to the 40S ribosomal subunit, modulated by eIFs.

bioRxiv · Aug 20, 2020Biophysics

eIF5B gates the transition from translation initiation to elongation

J. Wang, A. G. Johnson, C. P. Lapointe, J. Choi, A. Prabhakar, D.-H. Chen, A. N. Petrov, J. D. Puglisi

The paper investigates how eIF5B facilitates the transition from translation initiation to elongation in yeast using single-molecule fluorescence microscopy.

bioRxiv · Mar 23, 2019Biochemistry

RACK1 on and off the ribosome

A. G. Johnson, C. P. Lapointe, J. Wang, N. C. Corsepius, J. Choi, G. Fuchs, J. D. Puglisi

This study investigates RACK1's exchange dynamics on and off the ribosome using fluorescent labeling and biochemical analyses, showing its stable binding and limited conformational changes.

bioRxiv · Mar 21, 2019Biochemistry

De novo computational RNA modeling into cryoEM maps of large ribonucleoprotein complexes

K. Kappel, S. Liu, K. P. Larsen, G. Skiniotis, E. V. Puglisi, J. D. Puglisi, Z. H. Zhou, R. Zhao, R. Das

This paper introduces DRRAFTER, a method to model RNA-protein complexes from cryoEM maps, showing it can build accurate RNA coordinates in real-world scenarios.

bioRxiv · May 30, 2018Biophysics