Z. H. Zhou

Overcoming the preferred orientation problem in cryoEM with self-supervised deep-learning

This paper introduces spIsoNet, a deep-learning tool that improves 3D reconstruction isotropy and particle alignment in cryoEM by addressing the preferred orientation problem without altering specimen preparation.

TomoNet: A streamlined cryoET software pipeline with automatic particle picking on flexible lattices

TomoNet is a cryoET software pipeline with automatic particle picking and subtomogram averaging, achieving high-resolution 3D structures from flexible lattices.

Structure-Guided Mutagenesis Targeting Interactions between pp150 Tegument Protein and Small Capsid Protein Identify Five Lethal and Two Live Attenuated HCMV Mutants

The paper identifies five lethal and two live attenuated HCMV mutants by mutating pp150-capsid interactions, revealing critical residues for viral replication.

Encapsulation of AAVs into protein vault nanoparticles as a novel solution to gene therapy's neutralizing antibody problem

This study encapsulates AAVs into protein vault nanoparticles to bypass neutralizing antibodies, enabling effective gene therapy despite preexisting immunity.

Chemoproteomics reveals the epoxidase enzyme for the biosynthesis of camptothecin precursor strictosamide epoxide

This study identifies the epoxidase enzyme responsible for converting strictosamide to its epoxide form using a chemoproteomic approach in Ophiorrhiza pumila.

The universal suppressor mutation in the HSV-1 nuclear egress complex restores membrane budding defects by stabilizing the oligomeric lattice

The study identifies a suppressor mutation in HSV-1 NEC that stabilizes the hexagonal lattice, restoring membrane budding in various mutants.

CryoEM Reveals Oligomeric Isomers of a Multienzyme Complex and Assembly Mechanics

This study uses cryoEM to reveal the oligomeric isomers and assembly mechanics of PCC from Leishmania tarentolae, showing how subunit attachment affects enzymatic activity.

FAP106 is an interaction hub required for assembly of conserved and lineage-specific microtubule inner proteins at the cilium inner junction

This study identifies FAP106 as a critical interaction hub for assembling conserved and lineage-specific microtubule inner proteins at the cilium inner junction in Trypanosoma brucei.

The high-resolution structure of cell membranes revealed by in situ cryo-electron tomography

This study uses cryo-electron tomography to reveal the high-resolution 3D structure of human red blood cell membranes and their protein distribution.

Isotropic Reconstruction of Electron Tomograms with Deep Learning

This paper introduces IsoNet, a deep learning tool that reduces resolution anisotropy in cryoET data, improving structural interpretability without sub-tomogram averaging.

Molecular mechanism of TRPV2 channel modulation by cannabidiol

This study determines the structure of TRPV2 bound to cannabidiol, revealing a unique binding site and the role of the S4-S5 linker in channel gating.

Structural insights on TRPV5 gating by endogenous modulators

The paper determines TRPV5 structures with endogenous modulators PI(4,5)P2 and CaM, revealing their mechanisms of activation and inhibition.

De novo computational RNA modeling into cryoEM maps of large ribonucleoprotein complexes

This paper introduces DRRAFTER, a method to model RNA-protein complexes from cryoEM maps, showing it can build accurate RNA coordinates in real-world scenarios.