J. Rai

Conformational Changes Regulate Metal Coordination in the Catalytic Sites of Cas9

This study presents cryo-EM structures of AceCas9 at different states to show how conformational changes regulate metal coordination and catalytic activity.

Patterns and predictors of human-sloth bear conflict in Nepal: Implications for coexistence in unprotected forest landscapes

The paper analyzes human-sloth bear conflict in Nepal's unprotected forests, identifying risk factors and suggesting mitigation strategies through participatory mapping and education.

Molecular Mechanism of Active Cas7-11 in Processing CRISPR RNA and Interfering Target RNA

This study presents the cryo-EM structure of Cas7-11, revealing its mechanism for processing CRISPR RNA and cleaving target RNA.

Mmp14 is required for matrisome homeostasis and circadian rhythm in fibroblasts

This study shows that Mmp14 regulates circadian rhythms and matrisome homeostasis in tendon fibroblasts by controlling collagen fibril synthesis and crosslinking.

Pseudouridine-free Ribosome Exhibits Distinct Inter-subunit Movements

This study compares the structural differences between a pseudouridine-modified and pseudouridine-free ribosome, showing how the absence of pseudouridine affects inter-subunit movements and translation efficiency.

Age-Related Type I Collagen Modifications Reveal Tissue-Defining Differences Between Ligament and Tendon

This study compares collagen post-translational modifications in bovine tendons and ligaments to identify tissue-specific differences in cross-linking profiles and fibril size.

MITOCHONDRIAL GENOME REVEALS CONTRASTING PATTERN OF ADAPTIVE SELECTION IN TURTLES AND TORTOISES

The paper analyzes mitochondrial genomes of turtles and tortoises to compare adaptive selection patterns, finding stronger positive selection in turtles than tortoises.

Structure and Function of an in vivo Assembled Type III-A CRISPR-Cas Complex Reveal Critical Roles of Dynamics in Activity Control

The paper structures and functionally characterizes an in vivo assembled Type III-A CRISPR-Cas complex, revealing how RNA binding controls its enzymatic activity through protein dynamics.

An open interface in the pre-80S ribosome coordinated by ribosome assembly factors Tsr1 and Dim1 enables temporal regulation of Fap7

The paper determines the structure of an 80S-like ribosome assembly intermediate, revealing how Tsr1 and Dim1 coordinate to regulate Fap7 and enable quality control during ribosome maturation.

Physioxia stimulates extra-cellular matrix deposition and increases mechanical properties of human chondrocyte-derived tissue-engineered cartilage

This study shows that culturing human chondrocyte-derived cartilage under physiological oxygen tension increases extracellular matrix components and mechanical properties.