Julie Mathieu

Integrin-independent Tie2 activation using de novo designed proteins

This paper designs a de novo Tie2 binder that activates the Angiopoietin-Tie2 pathway without integrin engagement, showing improved therapeutic potential in a mouse model of ARDS.

Designed Minibinders Rewire Receptor Signaling to Enable Functional Human Myogenic Reprogramming

This paper demonstrates that AI-designed receptor modulators can drive efficient human fibroblast-to-muscle transdifferentiation by rewiring extracellular signaling.

Phosphoproteomics of Hypertrophic Cardiomyopathy Patient Myocardium and Novel hiPSC-CM Model Reveal Protein Kinase A as a Modulator of Microtubule Repolymerization

The paper identifies altered microtubule post-translational modifications in hypertrophic cardiomyopathy and shows protein kinase A modulates microtubule repolymerization in patient-derived hiPSC-CMs.

Molecular mechanisms of altered contraction with the β-myosin R403Q mutation in porcine ventricular muscle and a human stem cell-derived cardiomyocyte model.

This study investigates the R403Q mutation in beta-myosin heavy chain using porcine and human stem cell models, revealing altered contractile properties and mechanisms of hypercontractility and impaired relaxation.

Direct cell reprogramming by a designed agonist inducing HER2-FGFR proximity

This paper designs a synthetic ligand, H2F, that induces HER2-FGFR proximity to reprogram fibroblasts into myogenic cells and promote myogenic regeneration.

Human iPSC-derived salivary gland organoids model diabetic salivary gland dysfunction

This study develops human iPSC-derived salivary gland organoids that model diabetic dysfunction, showing impaired oxidative phosphorylation and FOXO1 nuclear localization in response to Metformin.

Identification of functional non-coding variants associated with orofacial cleft

This study identifies functional non-coding variants associated with orofacial cleft by combining GWAS, reporter assays, and stem cell modeling.

Designed NGF mimetics with reduced nociceptive signatures in neurons

The paper designs TrkA agonists that activate neuronal differentiation and neurite outgrowth without triggering pain-related gene expression.

Designed Soluble Notch Agonist Drives Human Ameloblast Maturation for Tooth Regeneration

The paper demonstrates that a designed soluble Notch agonist can drive iAM maturation without odontoblast contact, revealing DLX3's role in ameloblast-specific Enamelin and MMP20 expression.

High incidence and geographic distribution of cleft palate cases in Finland are associated with a regulatory variant in IRF6

The paper identifies a regulatory variant in IRF6 associated with increased cleft palate risk in Finland and shows it disrupts IRF6 enhancer function.

A critical role for heme synthesis and succinate in the regulation of pluripotent states transitions

This study shows that heme synthesis and succinate regulate pluripotent state transitions in ESCs by affecting signaling pathways and triggering 2C-like reprogramming.

Human iPSC Derived Enamel Organoid Guided by Single-Cell Atlas of Human Tooth Development

The paper establishes a single-cell atlas of human tooth development and uses it to guide the in vitro differentiation of ameloblasts from iPSCs, developing a mineralizing enamel organoid system.

Computer Designed PRC2 Inhibitor, EBdCas9, Reveals Functional TATA boxes in Distal Promoter Regions

The paper uses a computationally designed PRC2 inhibitor, EBdCas9, to identify functional TATA boxes in distal promoter regions and demonstrate their role in gene activation.

Suppression of unwanted CRISPR/Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

This paper introduces dead-RNAs (dRNAs) to suppress unwanted CRISPR/Cas9 off-target and HDR-corrected site cleavage, enabling precise genome editing.

Epigenomic and 3D genome architecture in naïve and primed human embryonic stem cell states

The paper compares epigenomic and 3D genome architecture between naive and primed human embryonic stem cells, showing differences in enhancer marking and chromatin domain organization.