K. Gevaert

Filter-aided extracellular vesicle enrichment (FAEVEr) for proteomics

This paper introduces FAEVEr, a method for enriching extracellular vesicles for proteomics, and shows it effectively isolates EVs from complex samples.

Seed Longevity is Controlled by Metacaspases

The paper identifies metacaspases as regulators of seed longevity by modulating CDC48 activity through proteolytic control of its adaptor PUX.

Shift in vacuolar to cytosolic regime of infecting Salmonella from a dual proteome perspective

The paper uses dual proteome profiling to study Salmonella Typhimurium-host interactions, finding DIA proteomics better identifies downregulated bacterial proteins during early infection.

Structure-function analysis of a calcium-independent metacaspase reveals a novel proteolytic pathway for lateral root emergence

This study solves the structure of a calcium-independent metacaspase and identifies inhibitors that block lateral root emergence in Arabidopsis.

Mapping the adaptor protein complex interaction network in Arabidopsis identifies P34 as a common stability regulator

The paper maps AP complex interactions in Arabidopsis, identifying P34 as a stability regulator of clathrin-mediated endocytosis and post-Golgi trafficking.

Mutation of Arabidopsis SME1 and Sm core assembly improves oxidative stress resilience

This study shows that mutating SME1 and Sm core assembly proteins in Arabidopsis enhances oxidative stress resilience by altering pre-mRNA splicing and activating a molecular stress response.

Novel assays monitoring direct glucocorticoid receptor protein activity exhibit high predictive power for ligand activity on endogenous gene targets

The paper introduces novel luciferase-based assays to monitor GR protein activity and shows that GR Ser211 phosphorylation is a reliable predictor of ligand effects on endogenous gene targets.

Proteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling

The paper uses CETSA MS to identify novel crosstalk between brassinosteroid and auxin signaling in Arabidopsis by analyzing the thermal and phospho-proteomes of the GSK3 inhibitor bikinin.

SAMBA controls the rate of cell division in maize development through APC/C interaction

This study shows that SAMBA regulates cell division rate in maize by interacting with APC/C, with different alleles causing varying growth defects due to altered protein function.

A Phelipanche ramosa KAI2 Protein Perceives enzymatically Strigolactones and Isothiocyanates

This study identifies PrKAI2d3 as a receptor in Phelipanche ramosa that perceives both strigolactones and isothiocyanates, showing its enzymatic activity enhances sensitivity to these signals.

The use of hybrid data-dependent and -independent acquisition spectral libraries empower dual-proteome profiling

This paper develops a hybrid spectral library approach combining data-dependent and in silico predicted data-independent acquisition proteomics to improve detection of host-pathogen proteomes during bacterial infections.

The deubiquitinating enzymes UBP12 and UBP13 negatively regulate the activity of the ubiquitin-dependent peptidases DA1, DAR1 and DAR2

The paper shows that UBP12 and UBP13 deubiquitinate DA1, DAR1, and DAR2, reducing their activity and affecting plant growth and development.

A simple approach for accurate peptide quantification in MS-based proteomics

This paper introduces a method to accurately quantify MS-ready peptides using a microfluidic UV/visible spectrophotometer, improving LC-MS/MS and protein quantification data consistency.

A well-ordered nanoflow LC-MS/MS approach for proteome profiling using 200 cm long micro pillar array columns

This paper introduces a long micro pillar array column for proteome profiling, showing improved protein identification and peak resolution compared to conventional columns.

A well-controlled BioID design for endogenous bait proteins

The paper introduces a controlled BioID design for endogenous p53 using CRISPR/Cas9 and base editors, showing improved proteomic results over ectopic expression.

DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis

The paper shows DET1 regulates H2Bub homeostasis in Arabidopsis by controlling the stability of a DUBm through interaction with DDA1.

Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome

The paper analyzes early proteome and phosphoproteome changes in Arabidopsis under mild mannitol stress and identifies AHA2 and CRRSP38 as novel regulators of shoot growth under osmotic stress.

Temperature-induced changes in wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms

The paper examines temperature-induced changes in wheat phosphoproteome, revealing temperature-regulated phosphoform interconversion and conserved regulatory mechanisms between leaf and spikelet.