J. Friml

The Unexpected Membrane Targeting of Marchantia Short PIN Auxin Exporters Illuminates Sequence Determinants and Evolutionary Significance

This study identifies the sequence determinant for PM localization in short PINs and explains the evolutionary shift from PM- to ER-localized PINs in land plants.

Super-resolution expansion microscopy in plant roots

This study develops and applies expansion microscopy to Arabidopsis root tissues, achieving improved resolution and visualizing subcellular structures.

Transport properties of canonical PIN-FORMED proteins and the role of the loop domain in auxin transport

This study quantifies the transport properties of canonical PIN-FORMED proteins and shows the loop domain's role in auxin transport beyond activation.

Guanylate cyclase activity of TIR1/AFB auxin receptors mediates rapid auxin responses

The paper shows TIR1/AFB auxin receptors have both adenylate and guanylate cyclase activities, mediating transcriptional reprogramming and rapid Ca2+-dependent growth responses.

Role of Dynamin-Related Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Plants

The paper investigates the role of DRP2s and SH3P2 in plant clathrin-mediated endocytosis, finding that DRP2s are recruited independently of BAR-SH3 proteins.

Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism

The paper visualizes NGR1 localization during root gravitropism and shows its role in facilitating rapid D6PK translocation to redirect auxin flux.

Mechanical forces in extendable tissue matrix orient cell divisions via microtubule stabilization in Arabidopsis

The paper shows mechanical forces from tissue extension stabilize microtubules to orient cell divisions in Arabidopsis roots after wounding.

Distinct functions of TIR1 and AFB1 receptors in auxin signalling

The paper compares the roles of TIR1 and AFB1 in transcriptional and rapid auxin responses, showing their distinct functions are due to specific protein properties, not subcellular localization.

Self-regulation of PIN1-driven auxin transport by cell surface-based auxin signaling in Arabidopsis

The paper identifies a cell surface-triggered auxin signaling mechanism that regulates PIN1-mediated auxin efflux and developmental patterning in Arabidopsis.

ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism

The paper identifies a mechanism where auxin signaling via ABP1-TMK1 receptor module stabilizes PIN2 to reinforce asymmetric auxin flow for root gravitropism.

A RAF-like kinase mediates a deeply conserved, ultra-rapid auxin response

The paper identifies a conserved RAF-like kinase mediating ultra-rapid auxin responses across land plants and algae, linking it to phosphorylation and fast physiological responses.

An ultra-fast, proteome-wide response to the plant hormone auxin

This study identifies an ultra-rapid auxin response system in Arabidopsis thaliana roots using phosphoproteomics, revealing over 1700 proteins modified within 30 seconds.

Angle-dependence and auxin asymmetry: towards the formulation of a universal theory of root gravitropism

This study integrates three gravitropism theories by showing angle-dependent auxin asymmetry and growth response in Arabidopsis roots.

Chemical inhibition of PIN auxin transporters by the anti-inflammatory drug Naproxen

This study identifies Naproxen as a chemical inhibitor of PIN auxin transporters in plants, revealing its mechanism of action through cellular, biochemical, and structural analyses.

User-friendly electron microscopy protocols for the visualization of biological macromolecular complexes in three dimensions: Visualization of planta clathrin-coated vesicles at ultrastructural resolution

The paper presents user-friendly electron microscopy protocols for visualizing plant clathrin-coated vesicles in 3D at nanometer resolution, along with a deep learning workflow for analysis.

Divergence of trafficking and polarity mechanisms for PIN auxin transporters during land plant evolution

This study investigates the divergence of PIN auxin transporter trafficking and polarity mechanisms across land plants, revealing differences between bryophytes and flowering plants.

Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole

The paper identifies CFS1 as an autophagy adaptor that connects autophagosomes with the ESCRT-I component VPS23 to form amphisomes, facilitating vacuolar delivery in plants.

SH3Ps recruit auxilin-like vesicle uncoating factors into clathrin-mediated endocytosis

The paper identifies SH3P1-3 as plant-specific homologues of endophilin/amphiphysin, showing they recruit auxilin-like uncoating factors into clathrin-mediated endocytosis in Arabidopsis.

Characterization of CAP1 and ECA4 adaptors participating in clathrin-mediated endocytosis

This study characterizes CAP1 and ECA4, two homologous ANTH-type clathrin adaptors in A. thaliana, showing their role in clathrin-mediated endocytosis and functional redundancy.

Developmental patterning function of GNOM ARF-GEF mediated from the plasma membrane

This study redefines the function of GNOM (GN) by showing its localization to the plasma membrane, distinct from clathrin-mediated endocytosis, and highlights its unique developmental role compared to its homologue GNL1.

ARF small GTPases in the developmental function mediated by ARF regulators GNOM and VAN3

The paper identifies ARLA1 as a functional partner of GNOM and VAN3 in branching angle control but finds no conclusive ARF partners for developmental patterning in Arabidopsis.

Proteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling

The paper uses CETSA MS to identify novel crosstalk between brassinosteroid and auxin signaling in Arabidopsis by analyzing the thermal and phospho-proteomes of the GSK3 inhibitor bikinin.

Clathrin-mediated endocytosis-independent function of VAN3 ARF GTPase Activating Protein at the plasma membrane

The paper investigates VAN3's function at the plasma membrane, showing it associates transiently with PM foci independent of clathrin and dynamin, and its localization depends on clathrin-mediated endocytosis.

The role of clathrin in exocytosis and the mutual regulation of endo- and exocytosis in plant cells

This study investigates clathrin's role in exocytosis and whether plant cells use homeostatic mechanisms to balance endo- and exocytosis, finding clathrin's involvement in exocytosis but no clear homeostatic regulation.

Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant Specific Components

The paper characterizes the proteome of Arabidopsis clathrin-coated vesicles, identifying conserved and plant-specific components involved in clathrin-mediated trafficking.

The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis

The paper identifies the TPLATE complex as a mediator of membrane bending in plant clathrin-mediated endocytosis, which occurs independently of actin and against high turgor pressure.

Rapid auxin-mediated phosphorylation of Myosin regulates trafficking and polarity in Arabidopsis

The paper identifies a rapid auxin-mediated phosphorylation of Myosin XI and MadB2 that regulates PIN transporter trafficking and polarity in Arabidopsis, revealing a non-canonical auxin response mechanism.

Auxin-triggered changes in the Arabidopsis root tip (phospho)proteome reveal novel root growth regulators

The paper identifies novel root growth regulators in Arabidopsis root tips through analysis of auxin-triggered (phospho)proteome changes.

PXC2, a polarized receptor kinase, functions to repress ground tissue cell divisions and restrict stele size

This study shows that PXC2, a polarized receptor kinase in Arabidopsis, redundantly represses endodermal cell divisions and stele expansion with IRK, and may influence auxin transport.

The PELOTA-HBS1 Complex Orchestrates mRNA Translation Surveillance and PDK1-mediated Plant Growth and Development

This study identifies the PELOTA-HBS1 complex's role in mRNA translation surveillance and PDK1-mediated plant growth by rescuing a pdk1 mutant in Arabidopsis.