Author

R. S. Johnston

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Bioengineering· 3

Signature Assays, Organisms & Methods

Click to filter · or open tag

Research Focus:drug response / drug discovery· 2 Approach:experimental assay· 2 Organism:yeast· 2 Approach:genetic perturbation / CRISPR· 1 Organism:human· 1 Organism:mouse· 1 Research Focus:protein folding & dynamics· 1 Assay:protein sequence· 1

Frequent Co-Authors

C. A. Naranjo· 3 shared J. C. Love· 3 shared N. C. Dalvie· 3 shared S. A. Rodriguez-Aponte· 3 shared H. D. Rao· 2 shared M. P. Rajurkar· 2 shared R. R. Lothe· 2 shared A. M. Biedermann· 1 shared

Activity by Year

2021-2022 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 43 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

3 shared papersBioengineering

C. A. Naranjo

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants

S. A. Rodriguez-Aponte, N. C. Dalvie, T. Y. Wong, R. S. Johnston, C. A. Naranjo, S. Bajoria, O. S. Kumru, K. Kaur, B. P. Russ, K. S. Lee, H. A. Cyphert, M. Barbier, H. D. Rao, M. P. Rajurkar, R. R. Lothe, , J. C. Love

bioRxiv · Sep 14, 2022Bioengineering

Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

N. C. Dalvie, C. A. Naranjo, S. A. Rodriguez-Aponte, R. S. Johnston, J. C. Love

The paper improves recombinant protein secretion quality and titer in Komagataella phaffii by modifying the N-terminus to enhance steric accessibility of the signal peptide.

bioRxiv · Jun 17, 2022Bioengineering

Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii

N. C. Dalvie, A. M. Biedermann, S. A. Rodriguez-Aponte, C. A. Naranjo, H. D. Rao, M. P. Rajurkar, R. R. Lothe, U. S. Shaligram, R. S. Johnston, L. E. Crowell, S. Castelino, M. K. Tracey, C. A. Whittaker, J. C. Love

This paper describes an engineered Komagataella phaffii strain for methanol-free production of SARS-CoV-2 RBD, achieving a 5x increase in secreted titer and enabling scalable manufacturing for vaccine development.

bioRxiv · Apr 15, 2021Bioengineering