S. Pagliara

Phage-induced efflux down-regulation boosts antibiotic efficacy

This study shows that phage presence reduces antibiotic efflux in bacteria, enhancing antibiotic efficacy and enabling lower concentrations for treatment.

Nutrient and salt depletion synergistically boosts glucose metabolism in individual bacteria

This study shows that nutrient and salt depletion in E. coli increases glucose accumulation rates at the translational and posttranslational levels, challenging the view that growth rate alone reflects metabolic status.

Fast bacterial growth reduces antibiotic accumulation and efficacy

The paper identifies fast-growing bacterial variants that avoid antibiotic accumulation and survive treatment without genetic mutations, challenging the current understanding of antibiotic resistance mechanisms.

Membrane permeability differentiation at the lipid divide

This study compares membrane permeability between archaeal and bacterial lipids using vesicles and finds that archaeal diether lipids are more permeable than bacterial diester lipids.

An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics

This study uses microfluidic platforms to show strong correlations between peptide antibiotics' physicochemical membrane activity and their cellular biological activity.

CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage

The paper determines the cryo-EM structure of the phage secretin pIV from f1 bacteriophage, models its function, and compares it to bacterial secretins.

Bacteriostatic antibiotics promote the evolution of CRISPR-Cas immunity

This study shows bacteriostatic antibiotics promote CRISPR-Cas immunity in Pseudomonas aeruginosa by slowing phage development and allowing more time for immune response.

Single cell label-free probing of chromatin dynamics during B lymphocyte maturation

This study uses Raman microscopy to analyze chromatin dynamics in B lymphocytes, showing spectral analysis can complement RNA-seq for studying gene activation during maturation.

Antibiotic transport kinetics in Gram-negative bacteria revealed via single-cell uptake analysis and mathematical modelling

This study quantifies antibiotic uptake in E. coli using microfluidics and microscopy, showing growth phase impacts drug accumulation more than transport pathways, and develops a mathematical model to predict kinetics.