U. F. Keyser

Nanopore translocation reveals electrophoretic force on non-canonical RNA:DNA double helix

The paper compares the electrophoretic translocation dynamics of RNA:DNA hybrids and DNA:DNA duplexes through nanopores, finding that RNA:DNA hybrids translocate faster despite similar contour lengths.

Multiplexed Digital Characterisation of Misfolded Protein Oligomers via Solid-State Nanopores

This paper introduces a single-molecule method using solid-state nanopores and DNA barcoding to detect and characterize misfolded protein oligomers, demonstrating its application to α-synuclein in Parkinson's disease.

Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination

This paper introduces a method using RNA nanotechnology and nanopores to quantitatively analyze alternative transcription termination at the single-molecule level.

Spatially Multiplexed Single-molecule Translocations through a Nanopore at Controlled Speeds

This paper introduces a method to control single-molecule translocations through a nanopore with spatial and temporal precision, achieving high-resolution DNA analysis.

Single-molecule sizing through nano-cavity confinement

This paper introduces a method called nano-cavity diffusional sizing (NDS) to measure the size of nanoscale particles and single biomolecules using residence times in nano-cavities.

2-photon-fabricated nano-fluidic traps for extended detection of single macromolecules and colloids in solution

This paper introduces nano-fluidic traps that increase detection times for single macromolecules and colloids in solution by providing local steric confinement.

Effective cell membrane tension is independent of polyacrylamide substrate stiffness

This study measures membrane tension in neurons and fibroblasts on different substrates, finding that effective membrane tension is independent of substrate stiffness and myosin II activity.

Nanopore microscope identifies RNA isoforms with structural colors

This study introduces ARTEMIS, a method using nanopore microscopy and structural colors to identify and quantify RNA isoforms without enzymatic amplification.

An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics

This study uses microfluidic platforms to show strong correlations between peptide antibiotics' physicochemical membrane activity and their cellular biological activity.

DNA Origami Voltage Sensors for Transmembrane Potentials with Single-Molecule Sensitivity

The paper develops DNA origami-based voltage sensors with single-molecule sensitivity, showing a 5% FRET change for 100 mV transmembrane potential shifts.

Measuring thousands of single vesicle leakage events reveals the mode of action of antimicrobial peptides

The paper uses high-resolution microscopy to study antimicrobial peptides' effects on vesicles, revealing their modes of action through detailed disruption measurements.

3D DNA structural barcode copying and random access

This paper demonstrates copying and random access of 3D DNA structural barcodes using modified PCR and nanopore measurements.

Antibiotic transport kinetics in Gram-negative bacteria revealed via single-cell uptake analysis and mathematical modelling

This study quantifies antibiotic uptake in E. coli using microfluidics and microscopy, showing growth phase impacts drug accumulation more than transport pathways, and develops a mathematical model to predict kinetics.

Secure data storage on DNA hard drives

The paper develops a DNA-based data storage system using Watson-Crick base pairing for secure, rewritable data storage on DNA hard drives.

Biophysical characterization reveals the similarities of liposomes produced using microfluidics and electroformation

The paper compares biophysical properties of GUVs produced by microfluidics and electroformation, finding similar lateral diffusion coefficients.

Monitoring G-quadruplex formation with DNA carriers and solid-state nanopores

This paper introduces a single-molecule Gq nanopore assay to detect G-quadruplexes and their folding kinetics without fluorescent labels.

Direct detection of molecular intermediates from first-passage times

The paper demonstrates how first-passage time distributions can reveal the number of intermediate states in complex molecular processes across different systems.

An integrated microfluidic platform for quantifying drug permeation across biomimetic vesicle membranes

The paper develops a microfluidic platform to measure drug permeability across biomimetic liposome membranes, demonstrating improved control and yield compared to existing methods.

Indole Pulse Signalling Regulates the Cytoplasmic pH of E. coli in a Memory-Like Manner

This study shows that indole pulses in E. coli regulate cytoplasmic pH in a memory-like manner, maintaining pH at 7.2 during stationary phase and protecting against stress.

Outperforming nature: synthetic enzyme built from DNA flips lipids of biological membranes at record rates

This paper describes a synthetic DNA enzyme that rapidly transports lipids between membrane leaflets, outperforming natural enzymes by three orders of magnitude.