T. B. K. Le

Molecular switching of a DNA-sliding clamp to a repressor mediates long-range gene silencing

The paper shows that KorB, a DNA-sliding clamp, switches to a repressor role with KorA to mediate long-range gene silencing on plasmid RK2.

Control of a gene transfer agent cluster in Caulobacter crescentus by transcriptional activation and anti-termination

The paper investigates how GafY and GafZ regulate the CcGTA gene cluster in Caulobacter crescentus through transcriptional activation and anti-termination mechanisms.

Cell cycle-dependent organization of a bacterial centromere through multi-layered regulation of the ParABS system

This study investigates the cell cycle-dependent regulation of the ParABS system in Bdellovibrio bacteriovorus, revealing how ParA:ParB ratios and chromosomal context influence centromere organization during its unique non-binary division.

Requirement for specific bacterial genome maintenance pathways in repair of C8-linked pyrrolobenzodiazepine (PBD) bi-aryl monomer-mediated DNA damage

This study investigates how C8-linked PBD bi-aryl monomers cause DNA damage in Caulobacter crescentus and identifies the repair mechanisms required for survival.

The CTP-binding domain is disengaged from the DNA-binding domain in a co-crystal structure of Bacillus subtilis Noc-DNA complex

The paper solves the structure of a truncated Bacillus subtilis Noc protein bound to NBS DNA, revealing disengagement between its CTP-binding and DNA-binding domains.

A CTP-dependent gating mechanism enables ParB spreading on DNA

This study identifies a CTP-dependent gating mechanism in ParB that regulates its nucleation, spreading, and recycling during chromosome segregation in Caulobacter crescentus.

CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc

This study shows that CTP regulates the membrane-binding activity of the nucleoid occlusion protein Noc in Bacillus subtilis by controlling its ability to form nucleoprotein complexes.

Chemical synthesis and purification of a non-hydrolyzable CTP analog CTPγS

The paper describes the chemical synthesis and purification of CTPγS, a non-hydrolyzable analog of CTP, to study CTPase enzymes.

Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic development

This study shows that during sporulation in Streptomyces venezuelae, chromosome arms transition from spatial separation to close alignment, mediated by ParB, SMC, and HupS.

Diversification of DNA-binding specificity via permissive and specificity-switching mutations in the ParB/Noc protein family

This study investigates how DNA-binding specificity diversifies in ParB and Noc proteins by identifying key residues and mutational paths that reprogram specificity.

ParB spreading on DNA requires cytidine triphosphate in vitro

This study shows that cytidine triphosphate (CTP) is required for ParB spreading on DNA in vitro, using purified proteins from Caulobacter crescentus.

Permissive zones for the centromere-binding protein ParB on the Caulobacter crescentus chromosome

The paper identifies additional parS sites and characterizes ParB's genomic distribution in Caulobacter crescentus, showing its role in chromosome segregation.

SMC Progressively Aligns Chromosomal Arms In Caulobacter crescentus But Is Antagonized By Convergent Transcription

This study shows that SMC aligns chromosomal arms in Caulobacter crescentus via ParB and that convergent transcription disrupts this alignment.