Author

T. Mielke

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3 Cell Biology· 2 Biophysics· 1 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Research Focus:cell type annotation· 2 Assay:cryo-EM· 2 Research Focus:drug response / drug discovery· 2 Research Focus:host-pathogen & immune response· 2 Organism:human· 2 Assay:proteomics· 2 Approach:statistical model· 2

Frequent Co-Authors

C. M. T. Spahn· 4 shared D. Schwefel· 3 shared A. Graziadei· 2 shared C. Gotthold· 2 shared J. Buerger· 2 shared J. Burger· 2 shared J. Rappsilber· 2 shared P. Scheerer· 2 shared

Activity by Year

2019-2022 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 34 mutual co-author links

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4 shared papersBiochemistry

C. M. T. Spahn

Mutual co-authors in cluster

Author profile →

7 papers

Recent·Most cited

An alpaca-derived nanobody neutralizes the SARS-CoV-2 omicron variant

N. Modhiran, S. M. Lauer, A. A. Amarilla, P. Hewins, S. I. L. van den Broek, Y. S. Low, N. Thakur, B. Liang, G. V. Nieto, J. Jung, D. Paramitha, A. Isaacs, J. de Sng, D. Song, J. Jorgensen, , D. Watterson

This paper identifies a nanobody that effectively neutralizes the SARS-CoV-2 Omicron variants BA.1 and BA.2, showing high affinity and pre-clinical promise.

bioRxiv · Dec 27, 2022 · PreprintMicrobiology

Time-resolved cryo-EM reveals early ribosome assembly in action

B. Qin, S. M. Lauer, A. Balke, C. Vieira-Vieira, J. Buerger, T. Mielke, M. Selbach, P. Scheerer, C. Spahn, R. Nikolay

The paper uses time-resolved cryo-EM to study early ribosome assembly in bacteria, revealing domain-wise and incremental assembly mechanisms and cooperative rRNA folding.

bioRxiv · Nov 12, 2022Biophysics

Structure and mechanism of a novel cytomegaloviral DCAF mediating interferon antagonism

V. T. K. Le-Trilling, S. Banchenko, D. Paydar, P. M. Leipe, L. Binting, S. Lauer, A. Graziadei, C. Gotthold, J. Burger, T. Bracht, B. Sitek, R. J. Lebbink, A. Malyshkina, T. Mielke, J. Rappsilber, , D. Schwefel

This study identifies E27 as a viral protein that targets STAT2 for degradation via CRL4 ubiquitin ligases, revealing its structural mechanism of interferon antagonism.

bioRxiv · May 5, 2022Biochemistry

Connecting the Dots: PHF13 and cohesin promote polymer-polymer phase separation of chromatin into chromosomes

F. Rossi, R. Buschow, L. V. Glaser, T. Schubert, H. Staege, A. Grimme, H. Will, T. Mielke, M. Vingron, A. Chiariello, S. Kinkley

This study shows PHF13 and cohesin work together to drive chromatin compaction into chromosomes through polymer-polymer phase separation.

bioRxiv · Mar 4, 2022 · PreprintCell Biology

Structures of active melanocortin-4 receptor--Gs-protein complexes with NDP-α-MSH and setmelanotide

N. A. Heyder, G. Kleinau, D. Speck, A. Schmidt, S. Paisdzior, M. Szczepek, B. Bauer, A. Koch, M. Gallandi, D. Kwiatkowski, J. Bürger, T. Mielke, A. Beck-Sickinger, P. W. Hildebrand, C. M. T. Spahn, , P. Scheerer

The paper presents cryo-EM structures of MC4R-Gs complexes with NDP-α-MSH and setmelanotide, revealing ligand-specific interactions and activation mechanisms.

bioRxiv · Apr 22, 2021Biochemistry

Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses

S. Banchenko, F. Krupp, C. Gotthold, J. Buerger, A. Graziadei, F. O' Reilly, L. Sinn, O. Ruda, J. Rappsilber, C. M. T. Spahn, T. Mielke, I. A. Taylor, D. Schwefel

This paper investigates how SIVmus Vpr interacts with CRL4DCAF1 to ubiquitylate SAMHD1, revealing structural and mechanistic insights into viral counteraction of host restriction factors.

bioRxiv · Dec 31, 2020Biochemistry

Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium

R. Brandt, T. Sell, M. Luethen, F. Uhlitz, B. Klinger, P. Riemer, C. Giesecke, S. Schulze, I. A. El-Shimy, D. Kunkel, B. Fauler, T. Mielke, N. Mages, B. G. Herrmann, C. Sers, , M. Morkel

The paper investigates how oncogenic KRAS and BRAF differentially activate ERK in colon cancer and intestinal epithelium, revealing cell type-specific effects and novel regulatory mechanisms.

bioRxiv · Feb 14, 2019Cell Biology