M. Selbach

Combining data independent acquisition with spike-in SILAC (DIA-SiS) improves proteome coverage and quantification

This paper introduces DIA-SiS, a method combining DIA with spike-in SILAC to improve proteome coverage and quantification, showing better performance than label-free approaches.

Time-resolved cryo-EM reveals early ribosome assembly in action

The paper uses time-resolved cryo-EM to study early ribosome assembly in bacteria, revealing domain-wise and incremental assembly mechanisms and cooperative rRNA folding.

Slice-PASEF: Maximising Ion Utilisation in LC-MS Proteomics

Slice-PASEF improves tandem MS sensitivity for low-sample proteomics, enabling applications like single-cell proteomics and drug screens.

Compound interaction screen on a photoactivatable cellulose membrane (CISCM) identifies drug targets

The paper introduces CISCM, a method to identify drug targets by immobilizing compounds on cellulose membranes and using proteomics, showing it can identify known targets quickly.

MYCN mediates cysteine addiction and sensitizes to ferroptosis

This study shows MYCN promotes cysteine addiction and ferroptosis resistance in neuroblastoma, identifying therapeutic targets for ferroptosis induction.

Proteome-wide quantitative RNA interactome capture (qRIC) identifies phosphorylation sites with regulatory potential in RBM20

The paper introduces qRIC to quantify RNA-binding protein-mRNA interactions and identifies phosphorylation sites in RBPs that regulate their function.

Novel LOTUS-domain proteins are organizational hubs that recruit C. elegans Vasa to germ granules

This study identifies MIP-1 and MIP-2 as C. elegans germ granule proteins that recruit Vasa homolog GLH-1 and are essential for granule formation and germ line development.

Neuroblastoma signalling models unveil combination therapies targeting feedback-mediated resistance

This study identifies feedback-mediated resistance to MEK inhibitors in neuroblastoma and shows that combining MEK with RAF or IGFR inhibitors can overcome resistance.

Insulin mRNA is stored in RNA granules in resting beta cells

The paper identifies RNA-binding proteins interacting with insulin mRNA in resting beta cells and shows glucose-induced remodeling of these interactions.

Monitoring mitochondrial translation by pulse SILAC

This study introduces a proteomic method to quantify mitochondrial-encoded proteins and reveals post-translational regulation of OXPHOS complexes.

Exploiting a PAX3-FOXO1-induced synthetic lethal ATR dependency for rhabdomyosarcoma therapy

This study identifies ATR as a synthetic lethal target in PAX3-FOXO1-driven rhabdomyosarcoma, showing ATR inhibition enhances PARP inhibitor efficacy and is tolerated in patients.

Cargo-specific recruitment in clathrin and dynamin-independent endocytosis

The paper identifies swiprosin-1 as a cargo-specific adaptor for the CG-pathway, showing its role in integrin internalization and breast cancer progression.

Cross-regulation of viral kinases with cyclin A secures shutoff of host DNA synthesis

The paper identifies how herpesvirus kinases interact with Cyclin A to inhibit host DNA synthesis during infection.

Evolutionary plasticity in the innate immune function of Akirin

This study identifies conserved and divergent roles of Akirin in innate immune gene regulation across species, revealing lineage-specific adaptations in its functional partners.

Picky: a simple method designer for targeted proteomics

Picky is a tool that automatically selects informative peptides for targeted proteomics methods, addressing the challenge of optimizing SRM and PRM assays.

Mutations In Disordered Regions Cause Disease By Creating Endocytosis Motifs

The paper identifies mutations in disordered regions that create dileucine motifs, leading to clathrin-dependent trafficking and disease.