Author

V. De Cesare

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 6

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Assay:proteomics· 4 Research Focus:drug response / drug discovery· 3 Organism:human· 3 Research Focus:cell signaling & trafficking· 1 Research Focus:cell type annotation· 1 Approach:deep learning· 1 Research Focus:protein folding & dynamics· 1

Frequent Co-Authors

A. Knebel· 4 shared C. Johnson· 3 shared F. Lamoliatte· 2 shared I. Cole· 2 shared M. Muqit· 2 shared O. Antico· 2 shared P. Davies· 2 shared S. M. Lange· 2 shared

Activity by Year

2017-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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4 shared papersBiochemistry

A. Knebel

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Author profile →

6 papers

Recent·Most cited

Discovery and characterization of non-canonical E2 conjugating enzymes

S. A. Abdul Rehman, E. Di Nisio, C. Cazzaniga, O. Antico, A. Knebel, C. Johnson, F. Lamoliatte, R. Negri, M. Muqit, V. De Cesare

The paper identifies and characterizes non-canonical E2 conjugating enzymes that ubiquitylate serine and threonine instead of lysine, revealing their target substrate and catalytic mechanism.

bioRxiv · Mar 5, 2023 · PreprintBiochemistry

Comprehensive approach to study branched ubiquitinchains reveals roles for K48-K63 branches inVCP/p97-related processes

S. M. Lange, M. R. McFarland, F. Lamoliatte, D. Kwasna, L. Shen, I. Wallace, I. Cole, L. A. Armstrong, A. Knebel, C. Johnson, V. De Cesare, Y. Kulathu

This paper develops a pipeline to study branched ubiquitin chains, identifies debranching enzymes, and reveals their roles in VCP/p97 processes and DNA damage responses.

bioRxiv · Jan 10, 2023Biochemistry

Elaboration of a MALDI-TOF Mass Spectrometry-based Assay of Parkin Activity and High-Throughput screening platform for Parkin Activators

R. Traynor, J. Moran, M. U. Stevens, A. Knebel, B. Behrouz, K. Merchant, C. J. Hastie, P. Davies, M. Muqit, V. De Cesare

The paper develops two MALDI-TOF MS-based methods to quantify Parkin E3 ligase activity in vitro, enabling high-throughput screening of Parkin activators.

bioRxiv · Mar 4, 2022 · PreprintBiochemistry

Characterization of protease activity of Nsp3 from SARS-CoV-2 and its in vitro inhibition by nanobodies

L. Armstrong, S. M. Lange, V. de Cesare, S. P. Matthews, R. S. Nirujogi, I. Cole, A. Hope, F. Cunningham, R. Toth, R. Mukherjee, D. Bojkova, F. Gruber, D. Gray, P. G. Wyatt, J. Cinatl, , Y. Kulathu

This study characterizes Nsp3 protease activity from SARS-CoV-2 and identifies nanobodies that inhibit PLpro with nanomolar affinity.

bioRxiv · Dec 9, 2020Biochemistry

Deubiquitinating Enzyme Amino Acid Profiling Reveals a Class of Ubiquitin Esterases

S. Virdee, V. De Cesare, D. Carbajo Lopez, P. D. Mabbitt, O. Antico, N. T. Wood

This study profiles DUBs for threonine esterase and lysine isopeptidase activity, revealing that the MJD class specifically exhibits ubiquitin esterase activity.

bioRxiv · May 11, 2020Biochemistry

The MALDI TOF E2/E3 ligase assay as an universal tool for drug discovery in the ubiquitin pathway

V. De Cesare, C. Johnson, V. Barlow, J. Hastie, A. Knebel, M. Trost

The paper introduces a MALDI TOF-based assay for high-throughput screening of E2/E3 ligase activity in the ubiquitin pathway, demonstrating its utility for drug discovery.

bioRxiv · Nov 29, 2017Biochemistry