Y. H. Grad

The inter-continental population dynamics of Neisseria gonorrhoeae

This study reconstructs the global population dynamics of Neisseria gonorrhoeae using phylogenomic analysis, revealing expanding effective population size and distinct transmission patterns between Norway and Victoria, Australia.

Bayesian modeling of the impact of antibiotic resistance on the efficiency of MRSA decolonization

This study uses a Bayesian mixed effects survival model to show that mupirocin resistance in MRSA strains reduces decolonization success, while other resistances and subject-specific factors do not.

Estimating the fitness cost and benefit of antimicrobial resistance from pathogen genomic data

This paper estimates the fitness cost and benefit of antimicrobial resistance using genomic data from Neisseria gonorrhoeae, showing that fluoroquinolone resistance could be safely used in a minority of cases.

Recent O-antigen diversification masks highly pathogenic STEC O104:H4

The paper identifies genomically similar, highly pathogenic STEC strains with diverse O-antigens, masking their potential risk due to rare serotypes.

Neisseria gonorrhoeae diagnostic escape from a gyrA-based test for ciprofloxacin susceptibility can lead to increased zoliflodacin resistance

The paper shows that Neisseria gonorrhoeae can escape ciprofloxacin susceptibility testing by reverting gyrA mutations, leading to zoliflodacin resistance.

Variation in supplemental carbon dioxide requirements defines lineage-specific antibiotic resistance acquisition in Neisseria gonorrhoeae

This study shows that metabolic variation in Neisseria gonorrhoeae, specifically CO2 dependence linked to a beta-carbonic anhydrase mutation, influences the likelihood of acquiring ciprofloxacin resistance.

Loci for prediction of penicillin and tetracycline susceptibility in Neisseria gonorrhoeae: a genome wide association study

This study identifies two chromosomal loci, penA_01 and rpsJ codon 57, along with plasmid loci blaTEM and tetM, that predict penicillin and tetracycline susceptibility in Neisseria gonorrhoeae with high specificity.

Emergence and evolution of antimicrobial resistance genes and mutations in Neisseria gonorrhoeae

This study traces the emergence and spread of antimicrobial resistance in Neisseria gonorrhoeae by analyzing genome sequences from multiple countries and time periods, revealing recombination events and antibiotic-driven mutations.

Increased antibiotic susceptibility in Neisseria gonorrhoeae through adaptation to the cervical environment

This study identifies loss-of-function mutations in efflux pumps as a mechanism for increased antibiotic susceptibility in Neisseria gonorrhoeae cervical isolates.

Efflux pump antibiotic binding site mutations are associated with azithromycin nonsusceptibility in clinical Neisseria gonorrhoeae isolates

The study confirms that specific MtrD efflux pump mutations in Neisseria gonorrhoeae are linked to increased azithromycin nonsusceptibility in clinical isolates.

Jointly modeling prevalence, sensitivity and specificity for optimal sample allocation

This paper introduces a joint Bayesian model to analyze lab validation and field survey data together, showing it improves prevalence and test characteristic estimates compared to separate analyses.

High-frequency phase-switching of modB methylase is associated with phenotypic ceftriaxone susceptibility in Neisseria gonorrhoeae

The paper shows high-frequency phase-switching of modB methylase in Neisseria gonorrhoeae is linked to decreased ceftriaxone susceptibility.

Increased power from bacterial genome-wide association conditional on known effects identifies Neisseria gonorrhoeae macrolide resistance mutations in the 50S ribosomal protein L4

This study uses conditional GWAS to identify a new azithromycin resistance mutation in Neisseria gonorrhoeae, demonstrating its prevalence and geographic spread.

Evaluation of parameters affecting performance and reliability of machine learning-based antibiotic susceptibility testing from whole genome sequencing data

This study evaluates factors affecting machine learning models' performance in predicting antibiotic resistance from whole genome sequencing data across multiple bacterial species.

Quantifying the surveillance required to sustain genetic marker-based antibiotic resistance diagnostics

The paper quantifies the surveillance needed to maintain the effectiveness of genetic marker-based antibiotic resistance diagnostics by identifying sampling and testing rates for early detection of novel resistance variants.

Comparative Tn-Seq reveals common daptomycin resistance determinants in Staphylococcus aureus despite strain-dependent differences in essentiality of shared cell envelope genes

This paper uses Tn-Seq to compare gene essentiality across multiple S. aureus strains, identifying common resistance determinants and strain-dependent differences under daptomycin exposure.

Impact of population structure in the design of RNA-based diagnostics for antibiotic resistance in Neisseria gonorrhoeae

This study evaluates RNA-based diagnostics for antibiotic resistance in Neisseria gonorrhoeae, finding that population structure affects transcriptional responses and identifying candidate transcripts for azithromycin resistance.

Photo-inactivation of Neisseria gonorrhoeae: A paradigm changing approach for combating antibiotic-resistant gonococcal infection

This study demonstrates that antimicrobial blue light (aBL) at 405 nm selectively inactivates antibiotic-resistant Neisseria gonorrhoeae without harming vaginal epithelial cells.

Azithromycin resistance through interspecific acquisition of an epistasis dependent efflux pump component and transcriptional regulator in Neisseria gonorrhoeae

The paper identifies epistatic interactions between mtrD and promoter mutations in Neisseria gonorrhoeae that contribute to azithromycin resistance through horizontal gene transfer.